Effect of Crown Restorations to Periodontal Markers in Healthy Subjects

Objectives: To investigate the possible influence of fixed dental prosthesis (FDP) procedures with/without gingival retraction to, MMP -1, MMP-8 and TNF-α; gingival crevicular fluid (GCF) levels in periodontally healthy subjects. Methods: A total of 8 periodontally healthy subjects who had at least 2 abutment teeth to prepare were included to the study. One of the abutment teeth per each patient with potassium-aluminum-sulfate braided retraction cord. GCF samples were obtained from two prepared teeth and one control tooth (negative control) before initiation of FDP treatment and repeated during the procedures (Day 2, 3, 7) and 1 month later after finishing the treatment. GCF concentrations of MMP-1,-8 and TNF-α; were determined by ELISA. Concentrations of the assayed proteins were analyzed by analysis of variance (ANOVA). Results: The MMP-1 concentrations showed slightly differences in between control, ret(-) and ret(+) groups when compared on the same sampling days, these differences were not found to be significant (p>0,05). The MMP-8 concentrations showed significant differences among study groups (P=0,017), but not among the sampling days (P>0,05). The TNF-α; concentrations showed significant differences in all study groups (P=0,014). In contrast to ret(-) and control groups, TNF-α; concentrations of ret(+) group seemed to be significantly different among sampling days (P=0,003). Conclusions: Within the limitations of present study, it may be suggested that well-fitted FDP procedures performed to periodontally healthy subjects do not permanently and negatory affect the periodontal tissue health.