IADR Abstract Archives

Synergistic anti-inflammatory and anti-oxidant activities of o-Cymen-5-ol and zinc chloride

Objectives: O-Cymen-5-ol is an isomer of thymol, an ingredient recognized as anti-oxidant and anti-microbial. To explore its full potential for use in oral health care applications, this study aims to evaluate o-Cymen-5-ol mediated inhibition of inflammation, oxidant reducing potential and promotion of cell migration using in vitro cellular models. Addition of zinc chloride was also investigated to assess potential additive or synergistic anti-inflammatory and anti-oxidant activities.

Methods: The anti-oxidant and anti-inflammatory activities of o-Cymen-5-ol and zinc chloride were assessed using RAW264.7 cells and Human Gingival Fibroblasts (HGF), respectively. Cultured cells were starved in low serum medium prior to treatment with P.gingivalis lipopolysaccride (PG-LPS) with or without testing agents. Release of nitric oxide radicals and Prostaglandin E2 (PGE2) were measured after 24 hours of treatment. Cell migration was assessed on HGF cells cultured with o-Cymen-5-ol at 2µM, 10µM and 50µM. A modified Boyden chamber assay was utilized to screen cell migration.

Results: O-Cymen-5-ol at 2-50µM inhibited PG-LPS-mediated release of Nitrite and PGE2 from cultured cells in a dose dependent manner. A 1.48 fold increase in gingival cell migration was observed with 50µM of o-cymen-5-ol relative to control. In addition, o-Cymen-5-ol and zinc chloride demonstrated strong synergistic anti-inflammatory and anti-oxidant activities on cultured cells.

Conclusion: The results indicate that o-Cymen-5-ol in combination with zinc chloride has good potential in promoting gingival health.


Continental European and Scandinavian Divisions Meeting
2011 Continental European and Scandinavian Divisions Meeting (Budapest, Hungary)
Budapest, Hungary
2011
467
Scientific Groups
  • Yang, Jingjun  ( GlaxoSmithKline, Weybridge, N/A, United Kingdom )
  • Myangar, Nisha  ( GlaxoSmithKline, Weybridge, N/A, United Kingdom )
  • Jeznach, Magdalena K.  ( Kingston University, Kingston Upon Thames, N/A, United Kingdom )
  • Jones, Lucy A.  ( Kingston University, Kingston Upon Thames, N/A, United Kingdom )
    • Poster Session
    Posters: Oral Health Research, Oral Medicine & Pathology, Micobiology/Immunology, and Cariology
    09/03/2011