IADR Abstract Archives

Effect of mineral trioxide aggregate on mesenchymal stem cells

Objectives: Mineral Trioxide Aggregate (MTA) has been used for both surgical and nonsurgical applications, such as root-end filling, perforation repair, resorptive defect repair, direct pulp capping, and apexification. The aim of our study was to evaluate the ability of MTA to support adhesion, proliferation and migratory ability of human bone marrow-derived mesenchymal stem cells (hMSC).

Methods: ProRoot MTA (Dentsply Tulsa, Tulsa, OK, USA) and a white Portland cement (Mapei, Italy) were mixed and left to set 24h. MSCs were cultured on the samples in the presence and absence of osteogenic medium and observed after 5 days by confocal scanning laser microscopy (CSLM) using the cytoskeleton marker phalloidin. Cell proliferation was evaluated by means of alamar blue assay after 1, 3, 5 and 7 days, using cells seeded on polystyrene culture wells as controls. In order to assess the effect on migratory ability of MSCs a transwell migration assay was performed for 18 h positioning MTA and Portland cements in 6-well plates and the cells in 8 μm pore inserts. The surface topography of cements was evaluated by atomic force microscopy.

Results: MSCs observed under CLSM showed proliferation and spread, forming a continuous layer on the upper surface of the MTA. Cells grown in osteogenic medium were bigger with a flat and polygonal shape. Cell proliferation was significantly higher on MTA than on Portland cement with an increase of 60% of the control after 1 and 3 days, while the peak of proliferation was reached after 5 days. Moreover, MTA was able to enhance cell migration significantly more than Portland cement. Finally, AFM images of MTA surface showed nanostructured features.

Conclusions: MTA was able to promote MSCs adhesion, proliferation and differentiation.

Acknowledgements: This study was supported by PRIN 2007M9YTFJ_003 and Rete Nazionale di Ricerca TissueNet RBPR05RSM2 from MIUR.


Continental European, Israeli, Scandinavian Divisions Meeting
2009 Continental European, Israeli, Scandinavian Divisions Meeting (Munich, Germany)
Munich Germany
2009
52
Scientific Groups
  • Di Caprio, Maria Patrizia  ( University of Naples "Federico II", Napoli, N/A, Italy )
  • D'anto', Vincenzo  ( Institute for Composite and Biomedical Materials - National Research Council, Napoli, N/A, Italy )
  • Spagnuolo, Gianrico  ( University of Naples "Federico II", Napoli, N/A, Italy )
  • Ametrano, Gianluca  ( University of Naples "Federico II", Napoli, N/A, Italy )
  • Ambrosio, Luigi  ( Institute for Composite and Biomedical Materials - National Research Council, Napoli, N/A, Italy )
  • Rengo, Sandro  ( University of Naples "Federico II", Napoli, N/A, Italy )
    • Poster Discussion Session
    Oral Poster Discussion I
    09/10/2009