Effects of Current Provisional Restoration Materials on Viability of Fibroblasts

Materials and Methods: The provisional restoration materials; Tempofit Duomix (Detax), Protemp 3 Garant (3M ESPE) and Revotek LC (GC Corporation) were prepared according to the manufacturers' instructions in standard teflon disks (2x5mm) and four samples were extracted in 7 ml of BME with 10% new born calf serum and 100 mg/ml penicillin/streptomycin for 24 hours. The L929 cells were plated (25.000 cells/ml) in wells of 96 well-dishes, and maintained in a humidified incubator for 24 hours at 37°C, 5% CO₂ and 95 % of air. After 24 hours incubation of the cells, the incubation medium was replaced by the immersed medium in which the samples were stored. Then L929 cells were incubated in contact with eluates for 24 hours. The cell mitochondrial activity was evaluated by the methyltetrazolium test (MTT). Twelve well used for each specimen and experiment repeated for two times. The data were submitted to the statistically analyzed by Mann-Whiney U tests.

Results: The results showed that, statistically Revotek LC and Protemp 3 Garant were not cytotoxic for L929 cells when compared to control group (p>0.05). Tempofit duomix was cytotoxic for L929 cells when compared to control group and other tested materials (p<0.05).

Conclusion: Taking into consideration the limitations of an in vitro study, our study indicate that provisional restoration materials might have cytotoxic effects and should be selected carefully for clinical applications.