IADR Abstract Archives

In-vitro CBCT Assessment of Scaffold's Reabsorption After Secondary CleftPalate Grafting

Objectives: Dental Pulp Stem Cells (DPSCs) can be used during the secondary grafting in complete cleft lip and palate patients in order to obtain the palate closure. Spongostan® was suggested as scaffold material in order to convey DPSCs into the bone defect. It's important to monitoring scaffold reabsorption and bone formation during the healing process. In this in-vitro study we want to investigate the possibility of evaluate by a Cone Beam Computed Tomography (CBCT) exam the presence of this scaffold material in different conditions: dry, wetted whit saline solution and with blood.

Methods: we build a test phantom utilizing a dry skull inserted into a plastic sheath lined with foam rubber and covered by a wetted sheet. We wet the scaffold material with a syringe inserting the needle through the plastic package and we insert it into the extraction socket of tooth 3.6 and into the left nasal cavity. We realize several CBCT exams, using the Newtom 3G® scanner (FOV of 6").

Results: It was possible to evaluate all the phantom bone structures. It was also possible to identify the tested material and to remark the image's differences between the blood and the saline solution wetted one. We can't localize the presence of the dry material.

Conclusions: CBCT exam expose the patient to a lower x-ray dose face to traditional multislice CT: this is really important in young patients with pathologies, like cleft lip and palate, that requires several radiological exams during the treatment. Our in-vitro results suggests that CBCT can be very useful in the assessment of scaffold reabsorption and bone formation during the bone healing process after the secondary grafting in complete cleft lip and palate patients and they encourage to replicate in-vivo this trial during a CBCT exam realized for orthodontic reasons.


Division: Continental European, Israeli, Scandinavian Divisions Meeting
Meeting: 2009 Continental European, Israeli, Scandinavian Divisions Meeting (Munich, Germany)
Location: Munich Germany
Year: 2009
Final Presentation ID: 371
Abstract Category|Abstract Category(s): Scientific Groups
Authors
  • Dalessandri, Domenico  ( Università degli studi di Torino - Dipartimento di scienze biomediche e oncologia umana, Torino, N/A, Italy )
  • Frongia, Gianluigi  ( Università degli studi di Torino - Dipartimento di scienze biomediche e oncologia umana, Torino, N/A, Italy )
  • Piancino, Maria G.  ( Università degli studi di Torino - Dipartimento di scienze biomediche e oncologia umana, Torino, N/A, Italy )
  • Paganelli, Corrado  ( Faculty of Dentistry, University of Brescia, Brescia, N/A, Italy )
  • Bracco, Pietro  ( Università degli studi di Torino - Dipartimento di scienze biomediche e oncologia umana, Torino, N/A, Italy )
  • SESSION INFORMATION
    Poster Discussion Session
    Oral Poster Discussion III
    09/12/2009