IADR Abstract Archives

Effect of 2-hydroxyethyl Methacrylate on B Cell Function

Objectives: B cells are the antibody producing cells of the immune system. Naive B cell express IgM on their surface and start producing primarily IgG once activated by an antigen. 2-hydroxyethyl methacrylate (HEMA) is a common monomer in dental bonding materials with allergenic properties. HEMA exposure has been shown to cause production of autoantibodies in mice. We wanted to investigate possible effects of HEMA on B cell antibody production.

Methods: Fresh buffy coat was supplied by healthy human volunteers after obtaining written informed consent and in accordance with permission received from the local ethical committee. From the buffy coat B cells were isolated by Ficoll density centrifugation and magnetic separation with anti-human CD19 labelled magnetic beads. B cells were then stimulated with HEMA for 6 days in the presence of pokeweed mitogen. IgG and IgM production was analysed with ELISA and proliferation determined by 3H Thymidine incorporation. The research was supported by the grant TUAGBG-20741 from the Faculty of Odontology (TUA), Gothenburg, Sweden.

Results: HEMA stimulated cells showed a concentration dependent 2-fold increase in production of IgG, a phenomenon not observed with IgM. No increased proliferation was observed.

Conclusion: HEMA exposure results in increased IgG production by peripheral blood B cells. This effect appears not to be caused by an increased B cell proliferation.


Continental European, Israeli, Scandinavian Divisions Meeting
2009 Continental European, Israeli, Scandinavian Divisions Meeting (Munich, Germany)
Munich Germany
2009
53
Scientific Groups
  • Andersson, Jennie  ( University of Gothenburg, Section for Oral Microbiology and Immunology, Gothenburg, N/A, Sweden )
  • Eklund, Christina  ( University of Gothenburg, Section for Oral Microbiology and Immunology, Gothenburg, N/A, Sweden )
  • Dahlgren, Ulf  ( University of Gothenburg, Section for Oral Microbiology and Immunology, Gothenburg, N/A, Sweden )
    • Poster Discussion Session
    Oral Poster Discussion I
    09/10/2009