IADR Abstract Archives
Capnocytophaga Detection at Species Level During Pregnancy Using MALDI-TOF MS
Objectives: Although the genus oral Capnocytophaga may increase their proportional growth in relation to hormonal changes and can lead to serious inflammations and adverse effects during pregnancy, the prevalence of those organisms among pregnant women is not thoroughly examined. This study aimed to determine the distribution of human Capnocytophaga at the species level using MALDI-TOF MS.
Methods: Subgingival Capnocytophaga isolates originated from 28 generally healthy and periodontitis-free pregnant women who were clinically three times during the pregnancy and two times after delivery. All isolates were preliminarily identified at the genus level based on phenotypic tests, then further examined with MALDI-TOF MS (Bruker Daltonics, Bremen, Germany) using the Microflex LT instrument and MALDI Biotyper software version 3.1. The specific cut-off scores were used to determine the genus level (1.700-1.999) and species level (≥2.000) identification.
Results: Out of 780 isolates, 351 (83.18%) were identified at the species level. The highest peak of the Capnocytophaga populations was at the second trimester (98 isolates), where MALDI-TOF MS identified 86.7% of them at the species level. C. ochracea was the most identified species in all five visits (76%), followed by C. sputigena (5%), C. haemolytica (2%) and C. granulosa (1%).
Conclusions: With the assistance of MALDI-TOF MS, the prevalence and distribution of Capnocytophaga species can be detected easily during pregnancy. C. ochracea in subgingival plaque was a common finding during pregnancy.
Methods: Subgingival Capnocytophaga isolates originated from 28 generally healthy and periodontitis-free pregnant women who were clinically three times during the pregnancy and two times after delivery. All isolates were preliminarily identified at the genus level based on phenotypic tests, then further examined with MALDI-TOF MS (Bruker Daltonics, Bremen, Germany) using the Microflex LT instrument and MALDI Biotyper software version 3.1. The specific cut-off scores were used to determine the genus level (1.700-1.999) and species level (≥2.000) identification.
Results: Out of 780 isolates, 351 (83.18%) were identified at the species level. The highest peak of the Capnocytophaga populations was at the second trimester (98 isolates), where MALDI-TOF MS identified 86.7% of them at the species level. C. ochracea was the most identified species in all five visits (76%), followed by C. sputigena (5%), C. haemolytica (2%) and C. granulosa (1%).
Conclusions: With the assistance of MALDI-TOF MS, the prevalence and distribution of Capnocytophaga species can be detected easily during pregnancy. C. ochracea in subgingival plaque was a common finding during pregnancy.