IADR Abstract Archives
Biological Properties of Calcium Phosphate Based Cement With Acetylsalicylic Acid
Objectives: The aim of this study was to evaluate the biocompatibility and antimicrobial effects of a newly developed calcium phosphate cement (CPC) with the addition of acetylsalicylic acid (ASA).
Methods: Hydroxyapatite doped with ions of strontium, copper, and zinc (multi-ion doped HAp, miHAp) powder was obtained by hydrothermal synthesis. Characterization of miHAp was done using X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray analysis (EDX). CPC was prepared by mixing calcinated miHAp with a liquid component (20% citric acid) containing three different concentrations of ASA. Five groups were studied: CPC; mineral trioxide aggregate (MTA); CPCA1 (1500 µg/g ASA); CPCA2 (3000 µg/g ASA); CPCA3 (4500 µg/g ASA). Biocompatibility was assessed on human dental pulp stem cells (hDPSCs) by MTT assay. Antimicrobial properties were evaluated by counting colony-forming units (CFUs) of Streptococcus mutans and Lactobacillus rhamnosus biofilms on material discs and in the medium surrounding discs.
Results: Characterization of the calcinated powder revealed particles ranging from a few hundred nanometers to approximately 2µm, presence of Sr and Cu ions as dopants, and α-tricalcium phosphate phase (α-TCP) as dominant. The results of biocompatibility show that compared to medium without extracts, cell viability was higher in the presence of two concentrations of CPCA1 and CPCA2 extracts, and all concentrations of CPCA3 extracts. Antimicrobial analysis indicated significantly lower CFUs on CPCA3 and MTA discs compared to CPC for both bacterial species (p<0.05). In the medium, antimicrobial effects against both bacterial species were observed for CPC and CPCA3 (p ≤ 0.0001).
Conclusions: Multi-ion doped cement based on calcium phosphate was successfully obtained. CPC with the addition of ASA increased cell proliferation. CPCA3 exhibited an antibiofilm effect against both bacterial species, while the antimicrobial effect was shown in the medium surrounding both CPC and CPCA3.
Methods: Hydroxyapatite doped with ions of strontium, copper, and zinc (multi-ion doped HAp, miHAp) powder was obtained by hydrothermal synthesis. Characterization of miHAp was done using X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray analysis (EDX). CPC was prepared by mixing calcinated miHAp with a liquid component (20% citric acid) containing three different concentrations of ASA. Five groups were studied: CPC; mineral trioxide aggregate (MTA); CPCA1 (1500 µg/g ASA); CPCA2 (3000 µg/g ASA); CPCA3 (4500 µg/g ASA). Biocompatibility was assessed on human dental pulp stem cells (hDPSCs) by MTT assay. Antimicrobial properties were evaluated by counting colony-forming units (CFUs) of Streptococcus mutans and Lactobacillus rhamnosus biofilms on material discs and in the medium surrounding discs.
Results: Characterization of the calcinated powder revealed particles ranging from a few hundred nanometers to approximately 2µm, presence of Sr and Cu ions as dopants, and α-tricalcium phosphate phase (α-TCP) as dominant. The results of biocompatibility show that compared to medium without extracts, cell viability was higher in the presence of two concentrations of CPCA1 and CPCA2 extracts, and all concentrations of CPCA3 extracts. Antimicrobial analysis indicated significantly lower CFUs on CPCA3 and MTA discs compared to CPC for both bacterial species (p<0.05). In the medium, antimicrobial effects against both bacterial species were observed for CPC and CPCA3 (p ≤ 0.0001).
Conclusions: Multi-ion doped cement based on calcium phosphate was successfully obtained. CPC with the addition of ASA increased cell proliferation. CPCA3 exhibited an antibiofilm effect against both bacterial species, while the antimicrobial effect was shown in the medium surrounding both CPC and CPCA3.