IADR Abstract Archives
Matrix Metalloproteinases Polymorphisms and Gene Expressions in Apical Periodontitis
Objectives: This study aimed to: (a) determine and compare the level of relative gene expression of matrix metalloproteinases (MMP)-2, -9, and their tissue inhibitor (TIMP2) in samples of AP and control healthy pulp tissues; (b) determine the frequencies of genotypes of single nucleotide polymorphisms (SNPs) in these genes in both groups; (c) determine the association of certain genotypes with gene expression levels.
Methods: The experimental group consisted of 50 AP samples harvested after apicoectomy, while control group consisted of 50 healthy pulp samples collected from teeth extracted due to orthodontic reasons. The level of relative gene expression was analyzed by reverse transcription and quantitative polymerase chain reaction (RT-qPCR), and genotyping for polymorphisms by restriction fragment length polymorphism analysis. Chi-square, Mann Whitney U test and logistic regression analysis were used for statistical data processing.
Results: The relative expression level of the MMP2 and MMP9 genes were statistically significantly higher in AP than in healthy controls (P=0.011, P=0.019, respectively). There was no statistically significant difference in the genotype distribution for the examined genes between the groups. Carriers of at least one copy of the wild-type gene for both MMP2 and MMP9 polymorphisms had higher expression values of this gene than homozygous carriers of the altered genes (P=0.037, P=0.042, P=0.031, P=0.046, respectively).
Conclusions: Matrix metalloproteinases are involved in the process of bone resorption in apical periodontitis, and polymorphisms in investigated genes change their expression in this study sample.
Methods: The experimental group consisted of 50 AP samples harvested after apicoectomy, while control group consisted of 50 healthy pulp samples collected from teeth extracted due to orthodontic reasons. The level of relative gene expression was analyzed by reverse transcription and quantitative polymerase chain reaction (RT-qPCR), and genotyping for polymorphisms by restriction fragment length polymorphism analysis. Chi-square, Mann Whitney U test and logistic regression analysis were used for statistical data processing.
Results: The relative expression level of the MMP2 and MMP9 genes were statistically significantly higher in AP than in healthy controls (P=0.011, P=0.019, respectively). There was no statistically significant difference in the genotype distribution for the examined genes between the groups. Carriers of at least one copy of the wild-type gene for both MMP2 and MMP9 polymorphisms had higher expression values of this gene than homozygous carriers of the altered genes (P=0.037, P=0.042, P=0.031, P=0.046, respectively).
Conclusions: Matrix metalloproteinases are involved in the process of bone resorption in apical periodontitis, and polymorphisms in investigated genes change their expression in this study sample.