IADR Abstract Archives
Bone Substitute Interaction With Gingival Cells
Objectives: In guided bone regeneration, in addition to the bone substitute, a membrane is used to allow bone regeneration and avoid the injured site colonization by soft tissues. Although soft tissues healing is essential from a functional and esthetic point of views, studies on the materials’ effects on soft tissue healing/regeneration are scant. The aim of this study was to investigate the effects of collagenated bone substitutes on gingival cells as compared to those of an anorganic substitute.
Methods: Primary human gingival cells were prepared from extracted third molars by the explant outgrowth method. Three bone-filling materials were used. Collagenated Gen-Os® and GTO® (Tecnoss Dental) and anorganic Bio-Oss® (Geistlich Pharma) were prepared according to the manufacturers’ instructions. The samples were dipped in MEM culture medium (20 mg/mL) through a membrane (Evolution®, Tecnoss Dental) for 24 hours. The effect of the extracts was evaluated on human gingival cell viability/proliferation (MTT test), while the effect on their colonization potential was investigated using a scratch assay after 24 hours.
Results: None of the materials was toxic to human gingival cells. Bio-Oss decreased cell proliferation after 3 and 7 days. Gingival cell proliferation significantly increased with collagenated materials. This increase was higher with GTO which significantly increased injured site colonization followed by Gen-Os. No increase of colonization was observed with Bio-Oss.
Conclusions: This study showed that bone substitutes extracts interact with gingival cells and affect their proliferation and the colonization of the injury site. These effects are dependent on the material nature. They are higher with collagen-containing materials than with the anorganic material. While our previous work demonstrated that collagenated substitutes induce angiogenesis and osteogenesis to a higher level than anorganic substitutes, these results are confirmed on gingival cells.
Methods: Primary human gingival cells were prepared from extracted third molars by the explant outgrowth method. Three bone-filling materials were used. Collagenated Gen-Os® and GTO® (Tecnoss Dental) and anorganic Bio-Oss® (Geistlich Pharma) were prepared according to the manufacturers’ instructions. The samples were dipped in MEM culture medium (20 mg/mL) through a membrane (Evolution®, Tecnoss Dental) for 24 hours. The effect of the extracts was evaluated on human gingival cell viability/proliferation (MTT test), while the effect on their colonization potential was investigated using a scratch assay after 24 hours.
Results: None of the materials was toxic to human gingival cells. Bio-Oss decreased cell proliferation after 3 and 7 days. Gingival cell proliferation significantly increased with collagenated materials. This increase was higher with GTO which significantly increased injured site colonization followed by Gen-Os. No increase of colonization was observed with Bio-Oss.
Conclusions: This study showed that bone substitutes extracts interact with gingival cells and affect their proliferation and the colonization of the injury site. These effects are dependent on the material nature. They are higher with collagen-containing materials than with the anorganic material. While our previous work demonstrated that collagenated substitutes induce angiogenesis and osteogenesis to a higher level than anorganic substitutes, these results are confirmed on gingival cells.