IADR Abstract Archives
Induction of Endothelial-Like Phenotype of Biobank-Stored Dental Pulp Stem Cells
Objectives: Dental pulp stem cells (DPCSs) are shown to be multipotent, being capable of differentiating into multiple lineages including endothelial cells under a defined condition. However, endothelial profile of induced DPSCs has not been well characterised, and it is not known if DPSC stored in a biobank for a prolonged period still maintain such capacity.
Methods: DPSC from 3 donors, isolated and stored in a biobank for over 5 years, were revived and induced to differentiate into endothelial-like cells using 3 different endothelial induction media: EGM-2, EGM-2MV and DMEM containing with 2% FBS and VEGF 50 ng/ml. Their endothelial profile and DPSC-induced vasculogenesis were evaluated using custom-made RT-qPCR array, tube formation assay, and ex ovo chorioallantoic membrane assay.
Results: After recovery, DPSCs still maintained the characteristic phenotype of mesenchymal stem cells (MSCs) including the expression of CD73, CD90, CD105 while lacking CD34, CD45, and HLA-DR. The cells were capable of differentiating into osteoblasts, adipocytes, and chondrocytes. Under endothelial induction, unexpectedly, DPSCs significantly downregulated various plausible endothelial markers including CD34, CD45, CD54, CD106, CD144. However, CD31 was highly upregulated regardless of the type of endothelial medium compared to the non-induced counterpart. Notably, the induced DPSCs exhibited functionalities resembling vascular endothelial cells with the promotion of vasculature formation.
Conclusions: The study suggests that despite limited expression of plausible endothelial markers, the endothelial-like cells derived from DPSCs stored long-term in the biobank still maintain the capacity of endothelial differentiation with vasculogenic functionality.
Methods: DPSC from 3 donors, isolated and stored in a biobank for over 5 years, were revived and induced to differentiate into endothelial-like cells using 3 different endothelial induction media: EGM-2, EGM-2MV and DMEM containing with 2% FBS and VEGF 50 ng/ml. Their endothelial profile and DPSC-induced vasculogenesis were evaluated using custom-made RT-qPCR array, tube formation assay, and ex ovo chorioallantoic membrane assay.
Results: After recovery, DPSCs still maintained the characteristic phenotype of mesenchymal stem cells (MSCs) including the expression of CD73, CD90, CD105 while lacking CD34, CD45, and HLA-DR. The cells were capable of differentiating into osteoblasts, adipocytes, and chondrocytes. Under endothelial induction, unexpectedly, DPSCs significantly downregulated various plausible endothelial markers including CD34, CD45, CD54, CD106, CD144. However, CD31 was highly upregulated regardless of the type of endothelial medium compared to the non-induced counterpart. Notably, the induced DPSCs exhibited functionalities resembling vascular endothelial cells with the promotion of vasculature formation.
Conclusions: The study suggests that despite limited expression of plausible endothelial markers, the endothelial-like cells derived from DPSCs stored long-term in the biobank still maintain the capacity of endothelial differentiation with vasculogenic functionality.