IADR Abstract Archives
Postpolymerization Affect Microbial Adhesion of Additively-Manufactured Resins for Definitive Restorations
Objectives: To investigate the influence of postpolymerization time (PPT) and atmosphere (PPA) on surface properties, protein adsorption, and microbial adhesion of two different types of additively manufactured (AM) resins for definitive restorations.
Methods: Two different types of commercially available AM resins for definitive restorations (UR and CR, respectively), were used to fabricate disk-shaped specimens by using digital light processing. Specimens were categorized into 8 groups based on the type (chemical composition), PPT (standard and extended), and PPA (air and nitrogen). After postpolymerization, all specimens were analyzed for surface roughness (Ra and Sa) and surface free energy (SFE). Protein adsorption, microbial (Streptococcus gordonii) attachment, and cytotoxicity were also investigated. Based on data distribution, non-parametric factorial analysis of variances and post-hoc analyses were performed with the level of significance (α) of 0.05.
Results: Ra and Sa values of CR group were higher than UR group, regardless of PPT or PPA (P<0.05). For UR, SFE under extended PPT was higher than that under standard PPT. CR showed higher SFE than UR under standard PPT. PPT and PPA interaction significantly affected the protein adsorption of resins (P<0.05). Under standard PPT, nitrogen significantly increased protein adsorption of resins compared with air. Interactions between the type and PPA, and the type and PPT had significant effects on microbial adhesion (P<0.05). For both resins, PPA or PPT significantly affected microbial adhesion on their surfaces. Cytotoxicity was not affected by the changes in PPT or PPA.
Conclusions: Surface properties, protein adsorption, and microbial attachment were affected by postpolymerization time, atmosphere, and chemical composition of the AM resins used for definitive restorations.
Methods: Two different types of commercially available AM resins for definitive restorations (UR and CR, respectively), were used to fabricate disk-shaped specimens by using digital light processing. Specimens were categorized into 8 groups based on the type (chemical composition), PPT (standard and extended), and PPA (air and nitrogen). After postpolymerization, all specimens were analyzed for surface roughness (Ra and Sa) and surface free energy (SFE). Protein adsorption, microbial (Streptococcus gordonii) attachment, and cytotoxicity were also investigated. Based on data distribution, non-parametric factorial analysis of variances and post-hoc analyses were performed with the level of significance (α) of 0.05.
Results: Ra and Sa values of CR group were higher than UR group, regardless of PPT or PPA (P<0.05). For UR, SFE under extended PPT was higher than that under standard PPT. CR showed higher SFE than UR under standard PPT. PPT and PPA interaction significantly affected the protein adsorption of resins (P<0.05). Under standard PPT, nitrogen significantly increased protein adsorption of resins compared with air. Interactions between the type and PPA, and the type and PPT had significant effects on microbial adhesion (P<0.05). For both resins, PPA or PPT significantly affected microbial adhesion on their surfaces. Cytotoxicity was not affected by the changes in PPT or PPA.
Conclusions: Surface properties, protein adsorption, and microbial attachment were affected by postpolymerization time, atmosphere, and chemical composition of the AM resins used for definitive restorations.