Comparison of Long Term Proliferation Profiles of DPSCs and BMSCs

Objectives: We assess and compare dynamics of proliferation of Dental pulp stem cells and Bone marrow stem cells after 20 passages (more than 30 cell doublings).
Methods: Dental pulp stem cells were isolated from routinely extracted third molars via enzymatic digestion (3 mg/mL collagenase type I and 4 mg/mL dispase) and cultured continuously up to passage 16. Cell count and population doubling were
evaluated at each passage. The enzymatic activity of telomerase and betagalactosidase
were assessed, as these are wellknown markers for cell senescence and aging. The following kits were used for these purpose, following the manufacturer`s instructions: Human TERT / Telomerase Reverse Transcriptase ELISA Kit (ELISAGenie, Dublin, Ireland) and ELISA Kit for Galactosidase Beta (GLb) (Cloud Clone Corp, Katy, TX, USA). Total protein amount was
previously identified in all samples using Nanodrop 1000 (Thermo Scientific).
Results: The results showed two peaks of significantly increased cellular proliferation rate at passages 6 and 12, with slight differences between cells from the first and 20th passages. The study indicates that dental pulp stem cells do not enter cell proliferation arrest phase after long-term in vitro cultivation, as there was no significant decrease in proliferation ability. However, alveolar bone stem cells showed a gradual decrease in proliferation after passage 15.
Conclusions: These findings contribute to a better understanding of the behavior and potential clinical applications of dental and alveolar bone stem cells in regenerative medicine.