IADR Abstract Archives
Saliva Collection: the Influence of Food Consumption and Toothbrushing on the Oral Microbiota
Objectives: In oral microbiota research, saliva is often the biological material of choice. To avoid bias in the results, it is necessary to take into account all factors that affect the saliva sample.
The aim of this project was to compare whether the condition of toothbrushing, food consumption, and varying time spacing between these factors can influence the bacterial profile of a saliva sample.
Methods: Unstimulated saliva was collected from six healthy respondents with excellent dental hygiene (exclusion and inclusion protocol) at intervals of (a) the morning before eating and toothbrushing with toothpaste, (b) immediately after eating and toothbrushing, (c) one hour after eating and toothbrushing, (d) two hours after eating and toothbrushing, (e) three hours after eating and toothbrushing.
Bacterial DNA from 30 samples was isolated using DNeasy PowerSoil Pro Kits (QIAGEN) and bacterial profiles were analyzed by PCR-denaturing gradient gel electrophoresis (DGGE).
Results: The PCR-DGGE profiles were compared. The pattern of bands was richest in collection (a) for four respondents, and in collection (b) for two respondents. The poorest bacterial profiles were observed in collection (c).
Conclusions: The study suggests that the time between toothbrushing and food consumption has an impact on the resulting bacterial profiles in saliva and may influence the results of the study. The above findings need further data on a larger number of respondents.
The aim of this project was to compare whether the condition of toothbrushing, food consumption, and varying time spacing between these factors can influence the bacterial profile of a saliva sample.
Methods: Unstimulated saliva was collected from six healthy respondents with excellent dental hygiene (exclusion and inclusion protocol) at intervals of (a) the morning before eating and toothbrushing with toothpaste, (b) immediately after eating and toothbrushing, (c) one hour after eating and toothbrushing, (d) two hours after eating and toothbrushing, (e) three hours after eating and toothbrushing.
Bacterial DNA from 30 samples was isolated using DNeasy PowerSoil Pro Kits (QIAGEN) and bacterial profiles were analyzed by PCR-denaturing gradient gel electrophoresis (DGGE).
Results: The PCR-DGGE profiles were compared. The pattern of bands was richest in collection (a) for four respondents, and in collection (b) for two respondents. The poorest bacterial profiles were observed in collection (c).
Conclusions: The study suggests that the time between toothbrushing and food consumption has an impact on the resulting bacterial profiles in saliva and may influence the results of the study. The above findings need further data on a larger number of respondents.