IADR Abstract Archives
The Influence of new Functionalized Scaffolds on Periodontal Ligament Cells Behaviour
Objectives: There is a need to develop shapeable multiphasic scaffolds with controlled architecture and bioactive properties suitable for periodontal regeneration. In this respect, this study aimed to assess the effect of new functionalized scaffolds elaborated by Direct-Writing Electrospinning (DWE) on the biological behavior of periodontal ligament cells (PDL).
Methods: Biphasic scaffolds based on polycaprolactone were elaborated using DWE technique; one of the two scaffold compartments contained hydroxyapatite nanoparticles (HAP) and the other the cementum protein 1 (CEMP1). After morphological characterizations, cell cytotoxicity, cell metabolic activity and cell colonization were evaluated using LIVE/DEAD staining, Alamar Blue assay and confocal imaging respectively. In addition, Alizarin Red staining and osteopontin (OPN) expression were studied to assess the mineralization potential of PDL cells after their contact with the experimental scaffolds.
Results: No cytotoxic effect was observed for all the tested scaffolds as shown by LIVE/DEAD staining. The results demonstrated that both HAP- and CEMP1- functionalized scaffolds were colonized by PDL cells and enhanced mineralization ability compared to unfunctionalized scaffolds; as revealed by alizarin red staining and OPN protein fluorescent expression using confocal imaging.
Conclusions:
The present in vitro results demonstrated that the functionalized scaffold (HAP and CEMP-1) enhanced PDL behaviour in terms of cell colonization and mineralization ability as compared to the unfunctionalized one. In the context of native tissue preservation and regeneration, these functionalized scaffolds deserve further development for potential clinical role in repairing and regenerating periodontal defects. Moreover, combining the high accuracy of DWE and the possibility of adding multiple bioactive agents paves the way for multi-tissue regeneration.
Methods: Biphasic scaffolds based on polycaprolactone were elaborated using DWE technique; one of the two scaffold compartments contained hydroxyapatite nanoparticles (HAP) and the other the cementum protein 1 (CEMP1). After morphological characterizations, cell cytotoxicity, cell metabolic activity and cell colonization were evaluated using LIVE/DEAD staining, Alamar Blue assay and confocal imaging respectively. In addition, Alizarin Red staining and osteopontin (OPN) expression were studied to assess the mineralization potential of PDL cells after their contact with the experimental scaffolds.
Results: No cytotoxic effect was observed for all the tested scaffolds as shown by LIVE/DEAD staining. The results demonstrated that both HAP- and CEMP1- functionalized scaffolds were colonized by PDL cells and enhanced mineralization ability compared to unfunctionalized scaffolds; as revealed by alizarin red staining and OPN protein fluorescent expression using confocal imaging.
Conclusions:
The present in vitro results demonstrated that the functionalized scaffold (HAP and CEMP-1) enhanced PDL behaviour in terms of cell colonization and mineralization ability as compared to the unfunctionalized one. In the context of native tissue preservation and regeneration, these functionalized scaffolds deserve further development for potential clinical role in repairing and regenerating periodontal defects. Moreover, combining the high accuracy of DWE and the possibility of adding multiple bioactive agents paves the way for multi-tissue regeneration.