Dental Whitening Effect of a Chewing-Gum With Solely E132

Objectives: To evaluate the instant optical whitening effect of a chewing-gum containing E132 (Indigotine) vs. a chewing-gum placebo after 2’30”
Methods: This was a single-center double-blind randomized parallel trial. To enter the trial, participants were required to have all their superior incisor teeth without restorations, no oral or systemic diseases, and no removable dentures. The 212 eligible participants, after a dental impression procedure, received a silicone mask with a calibrated hole at one upper incisor to apply a colorimeter (Vita^® Easyshade). They had to avoid oral hygiene for two hours before the appointment. The test chewing-gum (1.5g/piece) was added with 200ppm of E132, the placebo one was identical without pigments. The color of the teeth was detected before and after the mastication of one piece of chewing-gum for 2’30” with the colorimeter and the ΔWIO and ΔWI_D whiteness indexes were calculated as differences pre-post treatment. Statistical analysis was performed intragroup with the paired T-Test and between the groups with ANOVA. This trial was approved by the Ethical Committee (U.S.IRB2022PVM/01)
Results: All the participants completed the study, 119 females and 93 males (mean age 31.3±10.8). In the test group, the WIO and WI_D differences pre-post treatment (ΔWIO=1.85±3.49; ΔWI_D=1.13±1.58) were statistically significant (p≤0.001), instead in the placebo group they were not (p=NS). Both the ΔWIO and ΔWI_D after 2’30” were statistically different between the two chewing gums (p≤0.001). The acceptance was high, and none reported problems or side effects
Conclusions: This study suggests that the mastication of a chewing-gum containing solely E132 results in a perceptible whitening optical effect