IADR Abstract Archives
Salivary MMP-9 and SOD Levels After Non-Surgical Periodontal Treatment
Objectives: Matrix metalloproteinase 9 (MMP-9) is one of the major collagen-degrading enzymes in saliva associated with periodontitis and activated by oxidative stress. Superoxide dismutase (SOD) is an antioxidant enzyme acting against oxidative stress which increases in response to inflammation and tissue breakdown. The aim of the study was to assess the effect of non-surgical periodontal treatment (NSPT) on salivary MMP-9 and total SOD (T-SOD) levels in Stage-III Grade-B (P-III-B) and Grade-C (P-III-C) periodontitis patients.
Methods: A total of 49 subjects including 17 periodontally healthy (H), 16 P-III-B and 16 P-III-C were recruited for the study. At baseline the clinical periodontal parameters including plaque index, gingival index, bleeding on probing, probing depth and clinical attachment loss were recorded and the saliva samples were collected from all subjects. At 3 months after NSPT the clinical parameters were reevaluated, and the samples were recollected from only periodontitis groups. Salivary MMP-9 and T-SOD levels were analyzed by enzyme-linked immunosorbent assay.
Results: All periodontal parameters were higher in both periodontitis groups than H group at baseline (p<0.05) and improved 3 months after NSPT in both periodontitis groups (p<0.05). At baseline, salivary MMP-9 and T-SOD levels were higher in both periodontitis groups compared to H group (p<0.05) and significantly decreased after NSPT compared to baseline (p<0.05). Both salivary MMP-9 and T-SOD had diagnostic ability both for P-III-B (AUCs:0.764 and 0.774, respectively) and P-III-C (AUCs:0.772 and 0.733, respectively). Moreover, after adjusting for age, periodontitis groups were associated with both salivary MMP-9 and T-SOD levels (p< 0.05).
Conclusions: This study showed that elevated levels of salivary MMP-9 and T-SOD are associated with P-III-B and P-III-C. Moreover, after NSPT their levels were decreased.
Methods: A total of 49 subjects including 17 periodontally healthy (H), 16 P-III-B and 16 P-III-C were recruited for the study. At baseline the clinical periodontal parameters including plaque index, gingival index, bleeding on probing, probing depth and clinical attachment loss were recorded and the saliva samples were collected from all subjects. At 3 months after NSPT the clinical parameters were reevaluated, and the samples were recollected from only periodontitis groups. Salivary MMP-9 and T-SOD levels were analyzed by enzyme-linked immunosorbent assay.
Results: All periodontal parameters were higher in both periodontitis groups than H group at baseline (p<0.05) and improved 3 months after NSPT in both periodontitis groups (p<0.05). At baseline, salivary MMP-9 and T-SOD levels were higher in both periodontitis groups compared to H group (p<0.05) and significantly decreased after NSPT compared to baseline (p<0.05). Both salivary MMP-9 and T-SOD had diagnostic ability both for P-III-B (AUCs:0.764 and 0.774, respectively) and P-III-C (AUCs:0.772 and 0.733, respectively). Moreover, after adjusting for age, periodontitis groups were associated with both salivary MMP-9 and T-SOD levels (p< 0.05).
Conclusions: This study showed that elevated levels of salivary MMP-9 and T-SOD are associated with P-III-B and P-III-C. Moreover, after NSPT their levels were decreased.