IADR Abstract Archives
Transcriptomic Profiling of Periodontal Ligament Tissue Subjected to Orthodontic Force
Objectives: The biological response of the periodontal ligament (PDL) during orthodontic tooth movement (OTM) allows the teeth to move through the bone. This research investigates the transcriptomic profile of the PDL tissue of teeth subjected to an orthodontic force (OF), aiming to explain the molecular biological basis of OTM.
Methods: Patients seeking orthodontic treatment who needed orthodontic premolar extractions were invited to participate in this study. Patients were randomly assigned to two groups: control (CG) where the premolars were extracted before the application of OF and experimental (EG), where the premolars were extracted 28 days after the application of an OF of 50g, applied to the premolars by a 0.014 Ni-Ti arch wire. The total RNA was extracted from the PDL tissue and RNA-seq was subsequently performed. False discovery rates (FDR<0.05) and fold change≥1.5 thresholds were used to identify sets of differently expressed genes (DEGs). DEGs were further investigated to define their cellular and biological significance by Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) and network analysis.
Results: 17 and 23 patients were included in the EG and CG respectively. RNA-seq identified 172 DEGs in the EG compared to the CG, with 117 upregulated and 55 downregulated. According to the GO analysis, the DEGs in the EG were significantly enriched regarding biological processes associated with extracellular matrix organization and osteoclast differentiation, molecular functions associated with protein binding and cell components associated with focal adhesion. KEGG showed that the PI3K-Akt signal and axon guidance pathways are involved in OTM.
Conclusions: The gene expression profile of PDL tissue subjected to OF is significantly different from that of a CG without OTM. This is the first transcriptome study that provides whole transcriptome alteration in PDL tissue triggered by OF without using a split-mouth design.
Methods: Patients seeking orthodontic treatment who needed orthodontic premolar extractions were invited to participate in this study. Patients were randomly assigned to two groups: control (CG) where the premolars were extracted before the application of OF and experimental (EG), where the premolars were extracted 28 days after the application of an OF of 50g, applied to the premolars by a 0.014 Ni-Ti arch wire. The total RNA was extracted from the PDL tissue and RNA-seq was subsequently performed. False discovery rates (FDR<0.05) and fold change≥1.5 thresholds were used to identify sets of differently expressed genes (DEGs). DEGs were further investigated to define their cellular and biological significance by Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) and network analysis.
Results: 17 and 23 patients were included in the EG and CG respectively. RNA-seq identified 172 DEGs in the EG compared to the CG, with 117 upregulated and 55 downregulated. According to the GO analysis, the DEGs in the EG were significantly enriched regarding biological processes associated with extracellular matrix organization and osteoclast differentiation, molecular functions associated with protein binding and cell components associated with focal adhesion. KEGG showed that the PI3K-Akt signal and axon guidance pathways are involved in OTM.
Conclusions: The gene expression profile of PDL tissue subjected to OF is significantly different from that of a CG without OTM. This is the first transcriptome study that provides whole transcriptome alteration in PDL tissue triggered by OF without using a split-mouth design.