IADR Abstract Archives
Biocompatible Scaffolds With Osteoinductive Properties - in a Sheep Model
Objectives: For severe alveolar bone defects, autologous bone grafting has been considered the "gold standard"; however, the procedure has several disadvantages, including limited bone supply, resorption, donor site morbidity, infection, and bone graft rejection. In recent decades, bone tissue engineering has been shown to be a potential alternative to current bone augmentation techniques. The aim of the work was to study the microstructure of the maxillary sinus anterior wall using different biomaterials for regeneration, bacterial cellulose, and collagen, in a standardized sheep model.
Methods: A standardized bone defect on the anterior sinus wall was used. 3 groups of bone defects were formed, control group, group II closed with a collagen membrane, group III closed with a bone cover.
Microtomography scanning: A Skyscan 1176 X-ray computer microtomography was used. Bone samples were scanned together with calcium hydroxyapatite phantoms, diameter corresponding to the samples thickness for being compared with the histological results.
Histological study
Obtained bone tissue samples were decalcified by Trilon, subsequently paraffin embedded by Histomix. Histological sections of 5-7 µm thickness were made on a sledge microtome and stained with hematoxylin and eosin followed by general histopathological analysis.
Results: On 30th experimental, bone specimens showed defect replacement by different bone tissue with different differentiation degree. Group II bone defect showed bone fragments in the resorption state and signs of osteoclastic reaction. Group III bone defect was filled by newly formed bone tissue with minor signs of bone trabeculae resorption. The defect was filled with dense compact bone tissue, and roughly fibrous connective tissue showing trabeculae of newly formed bone tissue with a pronounced osteoblastic reaction.
Conclusions: Due to our sheep study model bone tissue regeneration follows a normal physiological type, starting from defect repair by granulation tissue with connective tissue bands and terminating by cell differentiation with a marked osteoblastic activity.
Methods: A standardized bone defect on the anterior sinus wall was used. 3 groups of bone defects were formed, control group, group II closed with a collagen membrane, group III closed with a bone cover.
Microtomography scanning: A Skyscan 1176 X-ray computer microtomography was used. Bone samples were scanned together with calcium hydroxyapatite phantoms, diameter corresponding to the samples thickness for being compared with the histological results.
Histological study
Obtained bone tissue samples were decalcified by Trilon, subsequently paraffin embedded by Histomix. Histological sections of 5-7 µm thickness were made on a sledge microtome and stained with hematoxylin and eosin followed by general histopathological analysis.
Results: On 30th experimental, bone specimens showed defect replacement by different bone tissue with different differentiation degree. Group II bone defect showed bone fragments in the resorption state and signs of osteoclastic reaction. Group III bone defect was filled by newly formed bone tissue with minor signs of bone trabeculae resorption. The defect was filled with dense compact bone tissue, and roughly fibrous connective tissue showing trabeculae of newly formed bone tissue with a pronounced osteoblastic reaction.
Conclusions: Due to our sheep study model bone tissue regeneration follows a normal physiological type, starting from defect repair by granulation tissue with connective tissue bands and terminating by cell differentiation with a marked osteoblastic activity.