IADR Abstract Archives
The Comparative Evaluation of an Experimental Dentin-Chip Added Pulp-Capping Material
Objectives: To compare the biocompatibility, inflammatory response and antimicrobial activity of an experimental material with different pulp-capping materials.
Methods: Five pulp-capping materials (ProRoot MTA/Dentsply, Biodentine/Septodont, Dycal/Dentsply, TheraCal LC/Bisco, calcium hydroxide/Saver-Pyrax) and an experimental material (calcium hydroxide+bovine dentin chip) were examined. The effects on cell viability were investigated via WST-1 assay in L929 cells. The inflammatory response was determined by the change in monocyte chemoattractant protein 1 (MCP-1/CCL2) and macrophage inflammatory protein-1α (MIP-1α/CCL3) levels. The antimicrobial activity against oral-microorganisms (Streptococcus mutans, Lactobacillus acidophilus and Enterococcus faecalis) was examined by agar-diffusion.
Results: The lowest cell viability was detected in ProRoot MTA and Dycal-exposed cells at 24 and 48 hours (p<0.05). The cell viability following the calcium hydroxide+bovine dentin chip was similar with Biodentine and control at 24 hours. The significant decrease was observed for cell viability of calcium hydroxide+bovine dentin chip group at 48 hours, but it was higher than ProRoot MTA and Dycal (p<0.05). The highest MCP-1 value was detected in ProRoot MTA and TheraCal LC-exposed cells at 24 and 48 hours compared to the control (p<0.05). The MIP-1α values in experimental groups were not different from the control. The MIP-1α level of calcium hydroxide+bovine dentin chip group was lower than ProRoot MTA and Biodentine at 48 hours (p<0.05). A limited inhibition zone against Streptococcus mutans was detected only in Dycal group.
Conclusions: The experimentally developed calcium hydroxide+bovine dentin chip combination showed competing properties and additional advantages compared to the existing pulp-capping materials. The calcium hydroxide+bovine dentin chip seems to have high potential to be considered as a biocompatible pulp-capping material.
Methods: Five pulp-capping materials (ProRoot MTA/Dentsply, Biodentine/Septodont, Dycal/Dentsply, TheraCal LC/Bisco, calcium hydroxide/Saver-Pyrax) and an experimental material (calcium hydroxide+bovine dentin chip) were examined. The effects on cell viability were investigated via WST-1 assay in L929 cells. The inflammatory response was determined by the change in monocyte chemoattractant protein 1 (MCP-1/CCL2) and macrophage inflammatory protein-1α (MIP-1α/CCL3) levels. The antimicrobial activity against oral-microorganisms (Streptococcus mutans, Lactobacillus acidophilus and Enterococcus faecalis) was examined by agar-diffusion.
Results: The lowest cell viability was detected in ProRoot MTA and Dycal-exposed cells at 24 and 48 hours (p<0.05). The cell viability following the calcium hydroxide+bovine dentin chip was similar with Biodentine and control at 24 hours. The significant decrease was observed for cell viability of calcium hydroxide+bovine dentin chip group at 48 hours, but it was higher than ProRoot MTA and Dycal (p<0.05). The highest MCP-1 value was detected in ProRoot MTA and TheraCal LC-exposed cells at 24 and 48 hours compared to the control (p<0.05). The MIP-1α values in experimental groups were not different from the control. The MIP-1α level of calcium hydroxide+bovine dentin chip group was lower than ProRoot MTA and Biodentine at 48 hours (p<0.05). A limited inhibition zone against Streptococcus mutans was detected only in Dycal group.
Conclusions: The experimentally developed calcium hydroxide+bovine dentin chip combination showed competing properties and additional advantages compared to the existing pulp-capping materials. The calcium hydroxide+bovine dentin chip seems to have high potential to be considered as a biocompatible pulp-capping material.