IADR Abstract Archives
Cellular Adhesion of Gingival Keratinocytes Treated With Platelet Rich Fibrin (PRF) on Titanium Surfaces- a Scanning Electron Microscope Study
Objectives: The soft tissue integration between the abutments of dental implants and the surrounding tissues is clinically central for the implant survival rate.
Methods: The growth media used to culture the Human gingival keratinocytes (HMK) were: elutes of titanium-PRF (T-PRF), leukocyte and platelet rich fibrin (L-PRF), and mammalian cell culture medium (SFM-X). The titanium disks used were titanium grade 4 (Ti4), titanium grade 5 (Ti5), and HA disks. The cell cultures to determine the proliferation rate and cell adhesion were performed at 37 °C in a CO2 incubator for 6 h and 24 h. For the scanning electron microscopy (SEM) imaging, the cells on different disks surface were chemically fixation with 5% glutaraldehyde, followed by a chemical dehydration in graded ethanol series (50%, 70%, and 98%) and allowed for overnight air-drying. The mounted disks on SEM specimen metal stubs were carbon-coated for 150 seconds. The LEO 1530 Gemini SEM (Carl Zeiss, Oberkochen, Germany) used to visualize the topographical features of the specimen.
Results: As a result, after 6 h incubation, further cell adhesion and spread had been observed on the Ti4 and Ti5 disks, in comparison to the HA group. However, the application of PRF enhanced the cell spread only on HA disks.
Conclusions: To conclude, epithelial cell adhesion and spread were titanium surface type-dependent and PRF has a regulatory role in the attachment.
Methods: The growth media used to culture the Human gingival keratinocytes (HMK) were: elutes of titanium-PRF (T-PRF), leukocyte and platelet rich fibrin (L-PRF), and mammalian cell culture medium (SFM-X). The titanium disks used were titanium grade 4 (Ti4), titanium grade 5 (Ti5), and HA disks. The cell cultures to determine the proliferation rate and cell adhesion were performed at 37 °C in a CO2 incubator for 6 h and 24 h. For the scanning electron microscopy (SEM) imaging, the cells on different disks surface were chemically fixation with 5% glutaraldehyde, followed by a chemical dehydration in graded ethanol series (50%, 70%, and 98%) and allowed for overnight air-drying. The mounted disks on SEM specimen metal stubs were carbon-coated for 150 seconds. The LEO 1530 Gemini SEM (Carl Zeiss, Oberkochen, Germany) used to visualize the topographical features of the specimen.
Results: As a result, after 6 h incubation, further cell adhesion and spread had been observed on the Ti4 and Ti5 disks, in comparison to the HA group. However, the application of PRF enhanced the cell spread only on HA disks.
Conclusions: To conclude, epithelial cell adhesion and spread were titanium surface type-dependent and PRF has a regulatory role in the attachment.