IADR Abstract Archives

Effects of L-PRF, a-PRF+ and i-PRF on Human Osteoblasts in Vitro.

Objectives: Different PRF protocols are used in clinical dentistry the last years. A positive effect of PRF in wound healing for soft tissues is known. However, hardly any literature comparing the new protocols for PRF (the A-PRF+ and i-PRF) with the original protocol of PRF (L-PRF), is present for osteoblasts and hard tissues.The aim of this study was to investigate the differences between Advanced Platelet Rich Fibrin (A-PRF+), Leukocyte Platelet Rich Fibrin (L-PRF) and injectable Platelet Rich Fibrin (i-PRF) in osteogenesis of a human osteoblast-like cell line in vitro.
Methods: A-PRF+, L-PRF and i-PRF was prepared from 6 male donors and cultured with 10 mL culture medium for 6 days. Osteoblasts from the osteoblast cell line (U2OS) were seeded in a density of 5 x 103 cells and cultured either with conditioned medium derived from the different PRF conditions or with fresh culture medium. At 5 different timepoints (0, 7, 14, 21, 28 days) the osteogenic capacity of the cells was assessed with an alizarin red staining to visualize mineralization. Next to that the calcium concentration, alkaline phosphatase activity was measured. With qPCR the expression of 6 osteogenic genes was measured (alkaline phosphatase, osteocalcin, osteonectin, ICAM-1, RUNX-2, Collagen 1a).
Results: A-PRF+ induced more mineralization and calcium after 28 days of culturing compared to the controls (p<0.001). i-PRF induced more calcium deposition compared to controls (p<0.05). No significant differences were found in cell proliferation and differentiation between the different conditions. However, there were significant differences between cell proliferation at different timepoints (0,7,14) in all conditions. Expression of RUNX-2 and osteonectin was lower in the PRF-cultures compared to control only at timepoint 7 and 14. Expression of οsteocalcin was higher in i-PRF and L-PRF at 21 days compared to 7 (p<0.05) and 14 (p<0.01) . ALP activity was significantly higher in the group cultured with the i-PRF medium (p< 0.01).
Conclusions: We found that only in the cultures with A-PRF+ more mineralization and calcium was present. This suggests that in the A-PRF+ condition the osteoblast-like cells are more differentiated to bone forming cells.

2021 Continental European and Scandinavian Divisions Meeting (Brussels, Belgium, Hybrid)
Brussels, Belgium, Hybrid
2021
0017
Mineralized Tissue
  • Kosmidis, Konstantinos  ( Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit , Amsterdam , Netherlands )
  • Ehsan, Karishma  ( Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit , Amsterdam , Netherlands )
  • Pitzurra, Luciano  ( Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit , Amsterdam , Netherlands )
  • Loos, Bruno  ( Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit , Amsterdam , Netherlands )
  • Jansen, Ineke  ( Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit , Amsterdam , Netherlands )
  • The authors do not have any financial interests, either directly or indirectly, in the products or information presented in the paper. The study was entirely financed by the Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), Ams
    Oral Session IN PERSON
    Mineralized tissue
    Thursday, 09/16/2021 , 02:45PM - 03:30PM