IADR Abstract Archives
Comparison of Gene Profiling of Individuals With Oligodontia and Normal Dentition
Objectives: Dentition is a complex adaptive system that is regulated by genetic, epigenetic, and environmental factors. Teeth development is directed by interactions between the ectoderm and mesenchyme and it is under strict genetic control. Oligodontia that is described as the absence of more than six permanent teeth excluding third molars can only be observed as isolated dental anomalies without any syndrome. However several studies were performed on mutational analysis of dental anomalies, there is no report for comprehensive transcriptomic analysis. Therefore, we focused to compare whole genome expression analysis to find target genes and pathways related to developmental tooth anomalies.
Methods: Gender and age matched five individuals with nonsyndromic oligodontia and 3 individuals with normal dentition participated to this study. Total RNA were isolated for whole genome gene expression analysis from the blood of the participants. cDNA was synthesized and HumanHT-12 v4 Expression Bead Chip Direct Hybridization Assay was used in Illumina iScan platform for over 47.000 probes. Data were subjected to quantile normalization before analysis.
Results: Findings demonstrated that whole genome expression analysis could discriminate participants who have nonsyndromic oligodontia and normal dentition. While 2956 probes were up-regulated (≥ 1.5 fold changes), 2894 probes were down-regulated in oligodontia cases. Hence, in addition to mutational analysis, RNA analysis of differentially expressed genes could provide further valuable information on the mechanisms of nonsyndromic oligodontia.
Conclusions: Further confirmation studies are required for genes including MSX1, AXIN2, EDARADD, PAX9, EDA, EDAR which were previously associated with non-syndromic tooth agenesis. Studies with higher number of participants might better clarify the role of specific genes directly related with oligodontia (Selcuk University BAP).
Methods: Gender and age matched five individuals with nonsyndromic oligodontia and 3 individuals with normal dentition participated to this study. Total RNA were isolated for whole genome gene expression analysis from the blood of the participants. cDNA was synthesized and HumanHT-12 v4 Expression Bead Chip Direct Hybridization Assay was used in Illumina iScan platform for over 47.000 probes. Data were subjected to quantile normalization before analysis.
Results: Findings demonstrated that whole genome expression analysis could discriminate participants who have nonsyndromic oligodontia and normal dentition. While 2956 probes were up-regulated (≥ 1.5 fold changes), 2894 probes were down-regulated in oligodontia cases. Hence, in addition to mutational analysis, RNA analysis of differentially expressed genes could provide further valuable information on the mechanisms of nonsyndromic oligodontia.
Conclusions: Further confirmation studies are required for genes including MSX1, AXIN2, EDARADD, PAX9, EDA, EDAR which were previously associated with non-syndromic tooth agenesis. Studies with higher number of participants might better clarify the role of specific genes directly related with oligodontia (Selcuk University BAP).