IADR Abstract Archives
Comparing Antimicrobial and Wound Healing Effects of Nano-Silver and Chlorhexidine
Objectives: To compare the antimicrobial and wound healing effects of a mouthwash containing silver nanoparticles with chlorhexidine mouthwash after oral surgery in rabbit models.
Methods: Microbial samples were collected from the oral cavity of 45 rabbit models. After sedation and local anesthesia, a 1-cm long, 1-mm deep wound was created in the lateral border of the tongue on the right side for all rabbits. To control the bleeding, the ends of the wound were sutured. After surgery, digital photographs were obtained from the wounds with standardized settings. The rabbits were then randomly divided into three groups: nano-silver group, chlorhexidine group, and negative control group. For the nano-silver group, a synthetic mouthwash containing 80.38 wt.% water, 9.80 wt.% glycerin, 9.80 wt.% nano-silver suspension with 400 ppm concentration, and 0.02 wt.% sodium saccharine was used. For the chlorhexidine group, 0.2% chlorhexidine solution was used. Four drops of each mouthwash were administered every eight hour for four days using a small syringe. The negative control group did not receive any mouthwash. Microbial samples were collected from all models each day for four days. Colony forming unit (CFU) counts were compared in post-operative and pre-operative samples. In addition, standardized digital photographs were taken each day from the wounds. The area of the wounds was compared post-operatively with the pre-operative values. Data was statistically analyzed using post hoc variance analysis and paired t-test (α=0.05).
Results: Mean CFU counts on post-operative days 3 and 4 were significantly different between the groups, with the nano-silver group showing the lowest values. Additionally, the mean areas of wound were significantly different in the experimental groups, indicating superior wound healing properties of the nano-silver mouthwash compared with chlorhexidine.
Conclusions: Synthetic mouthwash with 9.8 wt.% of nano-silver particles possesses better antimicrobial and wound healing properties compared with 0.2% chlorhexidine.
Methods: Microbial samples were collected from the oral cavity of 45 rabbit models. After sedation and local anesthesia, a 1-cm long, 1-mm deep wound was created in the lateral border of the tongue on the right side for all rabbits. To control the bleeding, the ends of the wound were sutured. After surgery, digital photographs were obtained from the wounds with standardized settings. The rabbits were then randomly divided into three groups: nano-silver group, chlorhexidine group, and negative control group. For the nano-silver group, a synthetic mouthwash containing 80.38 wt.% water, 9.80 wt.% glycerin, 9.80 wt.% nano-silver suspension with 400 ppm concentration, and 0.02 wt.% sodium saccharine was used. For the chlorhexidine group, 0.2% chlorhexidine solution was used. Four drops of each mouthwash were administered every eight hour for four days using a small syringe. The negative control group did not receive any mouthwash. Microbial samples were collected from all models each day for four days. Colony forming unit (CFU) counts were compared in post-operative and pre-operative samples. In addition, standardized digital photographs were taken each day from the wounds. The area of the wounds was compared post-operatively with the pre-operative values. Data was statistically analyzed using post hoc variance analysis and paired t-test (α=0.05).
Results: Mean CFU counts on post-operative days 3 and 4 were significantly different between the groups, with the nano-silver group showing the lowest values. Additionally, the mean areas of wound were significantly different in the experimental groups, indicating superior wound healing properties of the nano-silver mouthwash compared with chlorhexidine.
Conclusions: Synthetic mouthwash with 9.8 wt.% of nano-silver particles possesses better antimicrobial and wound healing properties compared with 0.2% chlorhexidine.