IADR Abstract Archives
Cytotoxicity of Commercial Hyaluronate-Based Aphthae Treatment Gels
Objectives: To evaluate the in vitro cytotoxic effects of topical hyaluronic acid-based aphthae treatment gels on human gingival fibroblasts.
Methods: Human gingival fibroblasts were seeded in 48-well plates and grown for 24 hours according to previously described and tested procedures. Four different hyaluronate-based gels (A - Bexident®AFT, B - GUM®AftaClear , C - GENGIGEL®, D - Aloclair®Plus) were tested. Five different gel/culture medium concentrations of each gel were incubated with cells for 15 minutes or 4 hours (n=8). Negative (medium) and positive (DMSO) controls were used. Cell viability was evaluated at 1 and 2 days after exposure, using a resazurin-based assay and results were converted to % of the negative control. Cell morphology was assessed through phase-contrast microscopy (PCM) and alterations were graded according to ISO10993-5:2009(E). Comparisons between groups were made through ANOVA with Tukey post-hoc tests, statistical significance was defined at p<0,05.
Results: In 15-minute evaluation Gels A and B presented higher viability than the other groups (p<0.05) with no cytotoxic effects. Gel C presented intermediate results for lower concentrations and was cytotoxic for 75% dilution. When exposed for 4h, all gels presented cytotoxicity for the 75% dilution. At lower concentrations (50%, 25% and 12.5%) gels A, B and C respectively, recovered viability up to 70%. Gel D had a significant cytotoxic effect at all timepoints and concentrations which was significantly lower than all other groups (p<0.05). PCM images confirmed viability results moderate to severe alterations in cells subjected to Gel D for all times, while for gels A-C only slight to mild changes were observed.
Conclusions: Of the tested products, Bexident®AFT and GENGIGEL® showed low to no cytotoxicity. GUM®AftaClear had intermediate cytotoxicity and Aloclair®, containing aloe barbadensis extract, showed a significantly higher cytotoxic potential in gingival fibroblasts comparing to the other tested gels.
Methods: Human gingival fibroblasts were seeded in 48-well plates and grown for 24 hours according to previously described and tested procedures. Four different hyaluronate-based gels (A - Bexident®AFT, B - GUM®AftaClear , C - GENGIGEL®, D - Aloclair®Plus) were tested. Five different gel/culture medium concentrations of each gel were incubated with cells for 15 minutes or 4 hours (n=8). Negative (medium) and positive (DMSO) controls were used. Cell viability was evaluated at 1 and 2 days after exposure, using a resazurin-based assay and results were converted to % of the negative control. Cell morphology was assessed through phase-contrast microscopy (PCM) and alterations were graded according to ISO10993-5:2009(E). Comparisons between groups were made through ANOVA with Tukey post-hoc tests, statistical significance was defined at p<0,05.
Results: In 15-minute evaluation Gels A and B presented higher viability than the other groups (p<0.05) with no cytotoxic effects. Gel C presented intermediate results for lower concentrations and was cytotoxic for 75% dilution. When exposed for 4h, all gels presented cytotoxicity for the 75% dilution. At lower concentrations (50%, 25% and 12.5%) gels A, B and C respectively, recovered viability up to 70%. Gel D had a significant cytotoxic effect at all timepoints and concentrations which was significantly lower than all other groups (p<0.05). PCM images confirmed viability results moderate to severe alterations in cells subjected to Gel D for all times, while for gels A-C only slight to mild changes were observed.
Conclusions: Of the tested products, Bexident®AFT and GENGIGEL® showed low to no cytotoxicity. GUM®AftaClear had intermediate cytotoxicity and Aloclair®, containing aloe barbadensis extract, showed a significantly higher cytotoxic potential in gingival fibroblasts comparing to the other tested gels.