IADR Abstract Archives
Saliva Viscosity of Frozen Saliva Samples of Healthy Respondents in Different Ages (Pilot Study)
Objectives: Human saliva has many different characteristics, which influences oral health considerably. The aim of the study was to measure the viscosity of frozen saliva samples of healthy subjects at different ages. Further, sialometric characteristics (flow rate, weight of saliva sediment and total salivary protein) have been determined.
Methods: The ethics committee of Witten/ Herdecke University approved the protocoll (98/2018). Twelve healthy subjects, who had no oral diseases and were not under medication that influences the water household, have been divided into two groups: younger (ages 18-25, n=6) and older (ages 70-85, n=6) subjects. Every subject collected saliva at three days in spring 2019 during period of ten minutes at four different times each day (T1: 7:15 a.m.; T2: 11:15 a.m.; T3: 3:15 p.m., T4: 7:15 p.m.). Saliva was then disposed, portioned, centrifuged and frozen. Flow rate, weight of sediment and total salivary protein was determined and viscosity was measured with a rheometer MCR 302 (Anton Paar, Graz,
Austria).
Results: Viscosity of saliva decreases with increasing shear forces. At the different loadings, viscosity of the older group showed significantly lower viscosity in daily profile than viscosity of the younger group. Weight of sediment in the daily profile of the older group, except T1, was considerably lower than the weight of sediment of the younger group. Total-protein weight and flow rate in the daily profile showed no significant differences between age groups.
Conclusions: Saliva of the older group in the daily profile showed less sediment (epithelial tissue and bacteria). It showed lower viscosity under pressure and was less able to glide than saliva of the younger group. It can be concluded that saliva of the older group loses the ability of ““washout”. The method of viscosity measuring of the frozen saliva samples demonstrated usable and convincing results so more extended studies could be carried out.
Methods: The ethics committee of Witten/ Herdecke University approved the protocoll (98/2018). Twelve healthy subjects, who had no oral diseases and were not under medication that influences the water household, have been divided into two groups: younger (ages 18-25, n=6) and older (ages 70-85, n=6) subjects. Every subject collected saliva at three days in spring 2019 during period of ten minutes at four different times each day (T1: 7:15 a.m.; T2: 11:15 a.m.; T3: 3:15 p.m., T4: 7:15 p.m.). Saliva was then disposed, portioned, centrifuged and frozen. Flow rate, weight of sediment and total salivary protein was determined and viscosity was measured with a rheometer MCR 302 (Anton Paar, Graz,
Austria).
Results: Viscosity of saliva decreases with increasing shear forces. At the different loadings, viscosity of the older group showed significantly lower viscosity in daily profile than viscosity of the younger group. Weight of sediment in the daily profile of the older group, except T1, was considerably lower than the weight of sediment of the younger group. Total-protein weight and flow rate in the daily profile showed no significant differences between age groups.
Conclusions: Saliva of the older group in the daily profile showed less sediment (epithelial tissue and bacteria). It showed lower viscosity under pressure and was less able to glide than saliva of the younger group. It can be concluded that saliva of the older group loses the ability of ““washout”. The method of viscosity measuring of the frozen saliva samples demonstrated usable and convincing results so more extended studies could be carried out.