IADR Abstract Archives

Gingival Crevicular Fluid MicroRNAs as Diagnostic Biomarkers for Periodontitis

Objectives: Periodontitis (PD) is a highly prevalent chronic inflammatory disease affecting tooth-supporting tissues and having a negative impact on overall health. Multiple mechanisms of pathogenesis of PD are epigenetically regulated and microRNAs (miRNAs) are considered as one of the key modulators that influence periodontal homeostasis. This study aimed to reveal PD-associated miRNAs in gingival tissue and gingival crevicular fluid (GCF) for subsequent use in non-invasive diagnostics of PD.
Methods: A total of 16 gingival tissue samples were used for genome-wide miRNA expression profiling. Following a thorough validation in an extended cohort of gingival tissue samples (N=80), miR-140-3p, -145-5p, -146a-5p, and -195-5p were selected for further analysis in GCF samples (N=210) by means of quantitative reverse transcription PCR.
Results: Microarray analysis revealed 140 miRNAs that were significantly upregulated in inflamed gingival tissues as compared to healthy controls. The levels of GCF miR-146a-5p were significantly lower in PD-affected individuals as compared to periodontally healthy participants (P<0.001). Severe forms of PD were associated with increased levels of GCF miR-140-3p and -145-5p and decreased levels of GCF miR-146a-5p (P<0.050). Moreover, the correlation between decreased levels of GCF miR-146a-5p and periodontal outcome parameters, indicating the worse clinical status of PD, were observed (P<0.010). The best diagnostic performance was demonstrated by a combination of the aforementioned miRNAs for diagnostics of both severe PD (AUC=0.709, P<0.001) and mild to moderate PD (AUC=0.612, P=0.018).
Conclusions: The current study revealed that miR-140-3p, -145-5p and -146a-5p were associated with PD and may be used as potential biomarkers for non-invasive diagnostics of PD.

2021 Continental European and Scandinavian Divisions Meeting (Brussels, Belgium, Hybrid)
Brussels, Belgium, Hybrid
2021
0048
Periodontal Research-Pathogenesis
  • Buragaite-staponkiene, Benita  ( Vilnius University , Vilnius , Lithuania )
  • Snipaitiene, Kristina  ( Vilnius University , Vilnius , Lithuania ;  National Cancer Institute , Vilnius , Lithuania )
  • Rovas, Adomas  ( Vilnius University , Vilnius , Lithuania ;  Vilnius University Hospital Zalgiris Clinic , Vilnius , Lithuania )
  • Puriene, Alina  ( Vilnius University , Vilnius , Lithuania ;  Vilnius University Hospital Zalgiris Clinic , Vilnius , Lithuania )
  • Punceviciene, Egle  ( Vilnius University , Vilnius , Lithuania ;  Vilnius University Hospital Santaros Clinics , Vilnius , Lithuania )
  • Butrimiene, Irena  ( Vilnius University , Vilnius , Lithuania ;  Vilnius University Hospital Santaros Clinics , Vilnius , Lithuania )
  • Jarmalaite, Sonata  ( Vilnius University , Vilnius , Lithuania ;  National Cancer Institute , Vilnius , Lithuania )
  • NONE
    European Regional Development Fund (project No. 01.2.2- LMT-K-718-01-0023), Research Council of Lithuania (LMTLT)
    Oral Session IN PERSON
    Periodontology
    Friday, 09/17/2021 , 01:30PM - 03:30PM