IADR Abstract Archives
Human Osteoblasts Proliferative Response to a Novel Photodynamic Therapy Protocol
Objectives: The success of photodynamic therapy mediated by the topical application of 5-aminolevulinic acid and light irradiation (ALA-PDT) in the treatment of neoplastic tissues encouraged the production of a novel gel, specially formulated for oral applications. We have shown the effectiveness of ALA-PDT for inactivating, in vitro, gram positive and negative bacteria. Despite the proven strong antibacterial and antifungal effect, which makes it an ideal product for the treatment of periodontitis and peri-implantitis, we have no information regarding its potential effects on bone tissue.
The aim of the present work was to evaluate, in vitro, the human osteoblast proliferation in response to ALA-PDT.
Methods: Osteoblasts, explanted from human mandible bone fragments, were plated (6x103 cells/well) and treated after 24h with different concentrations (10%, 50%, 100% v/v) of ALADENT (5-aminolevulinic acid at 5%, commercialized as ALADENT –-Alphastrumenti -) for 45 minutes. Subsequently, the cultures were irradiated with 7 minutes of 630 nm LED at an intensity of 380mW/cm2 (ALA-PDT), compared to either unexposed cells (CTRL) and cells exposed to LED alone (LED). The proliferation study was carried out by CellTiter 96-assay (Promega) at 48h and 72h after the treatments. Statistical analysis was performed by T-test with a statistical significance established at p<0.05.
Results: Following 48h of incubation, a significant increased cell proliferation (+22%) was observed only in cells subjected to ALA-PDT (100% v/v) respect to the osteoblast cultures either unexposed or exposed to LED alone. At 72h cells treated with LED alone, and those exposed to ALA-PDT with any concentrations(10%, 50%, 100% v/v) showed a significant increased proliferation, respect to unexposed control. In particular 100% ALA-PDT demonstrated an increase as 257% of cell proliferation.
Conclusions: Although further studies are needed, our data, for the first time, shed light on a new potential mechanism of 5-aminolevulinic acid action on osteoblast proliferation process.
The aim of the present work was to evaluate, in vitro, the human osteoblast proliferation in response to ALA-PDT.
Methods: Osteoblasts, explanted from human mandible bone fragments, were plated (6x103 cells/well) and treated after 24h with different concentrations (10%, 50%, 100% v/v) of ALADENT (5-aminolevulinic acid at 5%, commercialized as ALADENT –-Alphastrumenti -) for 45 minutes. Subsequently, the cultures were irradiated with 7 minutes of 630 nm LED at an intensity of 380mW/cm2 (ALA-PDT), compared to either unexposed cells (CTRL) and cells exposed to LED alone (LED). The proliferation study was carried out by CellTiter 96-assay (Promega) at 48h and 72h after the treatments. Statistical analysis was performed by T-test with a statistical significance established at p<0.05.
Results: Following 48h of incubation, a significant increased cell proliferation (+22%) was observed only in cells subjected to ALA-PDT (100% v/v) respect to the osteoblast cultures either unexposed or exposed to LED alone. At 72h cells treated with LED alone, and those exposed to ALA-PDT with any concentrations(10%, 50%, 100% v/v) showed a significant increased proliferation, respect to unexposed control. In particular 100% ALA-PDT demonstrated an increase as 257% of cell proliferation.
Conclusions: Although further studies are needed, our data, for the first time, shed light on a new potential mechanism of 5-aminolevulinic acid action on osteoblast proliferation process.