IADR Abstract Archives
Antibiofilm Activity of a Brushing Solution in Peri-Implant Biofilm Model
Objectives: To evaluate the potential antibiofilm effect of a brushing solution in an in-vitro peri-implant biofilm model developed on titanium surfaces.
Methods: Titanium discs (Ti-SLA) were pre-treated with a brushing solution (NitrAdine®, test), warm water (negative control) and chlorhexidine and cetylpyridinium chloride (CHX/CPC, positive control). An in-vitro validated peri-implant biofilm model (with Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Aggregatibacter Actinomycetemcomitans, Porphyromonas gingivalis and Fusobacterium nucleatum) was developed on the pre-treated surfaces. The discs were treated and re-incubated each 24 h until reach 72 h.
Biofilms (24 h, 48 h and 72 h) were analyzed by confocal laser scanning microscopy (CLSM), scanning electronic microscopy and quantitative polymerase chain reaction (qPCR) (viable colony forming units, CFU, per milliliter). A generalized linear model was constructed to determine the effect of the tested products on viable bacterial counts.
Results: Regarding CLSM analyses of biofilm formation and development, Ti-SLA discs pre-treated with test product demonstrated: a) the largest reductions in live/dead cell ratio, being statistically significant between 24-48 h and 24-72 h; and b) a more stable thickness over time, when compared with positive and negative controls.
qPCR analysis showed, after 24 h of re-incubation, lower counts of S. oralis and A. actinomycetemcomitans on Ti-SLA discs pre-treated with test product, when compared with negative control (p<0.05). After 48 h of re-incubation, differences were found between Ti-SLA discs exposed to test solution or negative control, for counts of S. oralis, A. naeslundii, V. parvula and P. gingivalis (p<0.05). After 72 h of re-incubation, test solution showed lower counts for S. oralis, A. naeslundii, V. parvula, P. gingivalis and F. nucleatum, when compared with negative control (p<0.05).
Conclusions: The tested brushing solution can impact the development of in-vitro peri-implant biofilms on titanium surfaces, in a biofilm model, by affecting its structure and vitality.
Methods: Titanium discs (Ti-SLA) were pre-treated with a brushing solution (NitrAdine®, test), warm water (negative control) and chlorhexidine and cetylpyridinium chloride (CHX/CPC, positive control). An in-vitro validated peri-implant biofilm model (with Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Aggregatibacter Actinomycetemcomitans, Porphyromonas gingivalis and Fusobacterium nucleatum) was developed on the pre-treated surfaces. The discs were treated and re-incubated each 24 h until reach 72 h.
Biofilms (24 h, 48 h and 72 h) were analyzed by confocal laser scanning microscopy (CLSM), scanning electronic microscopy and quantitative polymerase chain reaction (qPCR) (viable colony forming units, CFU, per milliliter). A generalized linear model was constructed to determine the effect of the tested products on viable bacterial counts.
Results: Regarding CLSM analyses of biofilm formation and development, Ti-SLA discs pre-treated with test product demonstrated: a) the largest reductions in live/dead cell ratio, being statistically significant between 24-48 h and 24-72 h; and b) a more stable thickness over time, when compared with positive and negative controls.
qPCR analysis showed, after 24 h of re-incubation, lower counts of S. oralis and A. actinomycetemcomitans on Ti-SLA discs pre-treated with test product, when compared with negative control (p<0.05). After 48 h of re-incubation, differences were found between Ti-SLA discs exposed to test solution or negative control, for counts of S. oralis, A. naeslundii, V. parvula and P. gingivalis (p<0.05). After 72 h of re-incubation, test solution showed lower counts for S. oralis, A. naeslundii, V. parvula, P. gingivalis and F. nucleatum, when compared with negative control (p<0.05).
Conclusions: The tested brushing solution can impact the development of in-vitro peri-implant biofilms on titanium surfaces, in a biofilm model, by affecting its structure and vitality.