IADR Abstract Archives
Periodontopathogens on the Implant Temporary Crowns- a Pilot Study
Objectives: Periimplant disease is the result of an imbalance between host response and bacterial load. This infection is attributed to the gram-anaerobic microflora, most of them also associated with periodontal disease as Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, Fusobacterium sp., Prevotella intermedia, Aggregatibacter actinomycetemcomitans, Staphylococcus aureus, and Candida albicans. The present study aimed to identify the presence of the main types of periodontopathogen microorganisms attributed to the periimplant disease on temporary crowns of the implant-supported restorations.
Methods: Biological materials were collected from the abutment surfaces and implant supported temporary restorations. Bacterial DNA was extracted using sodium hydroxide based chemical protocol and the presence of specific bacteria was evaluated by means of polymerase chain reaction (PCR), using previously designed species specific primers. The temporary crowns on temporary abutments were used for conditioning and contouring the emergence profile on peri-implant soft tissue. Provisionals were made from composite materials and were used for 3-6 months before the insertion of the final restoration.
Results: In the analysed biomaterial from 13 temporary crowns, Fusobacterium spp dominated (in 84.6% samples), followed by Porphyromonas gingivalis (in 61.5% samples). Aggregatibacter actinomycetemcomitans and Tannerella forsythia were found in 38.5% samples and rarely present were Treponema denticola and Prevotella intermedia (in 7.7% samples). In most cases (63.6%), Fusobacterium spp were identified together with Porphyromonas gingivalis. Samples mostly contained 4 or 5 periodontopathogens (together in 69.5% cases), and in 7.7% of cases all examined pathogens were identified.
Conclusions: The results revealed the presence of periodontopathogens on analysed surfaces. Precise identification of different types of periodontopathogens in bacterial colonization of implant abutments and gingival surfaces of the crowns is important for early detection of the infection and consequently for the prevention and proper treatment of periimplant disease. Further research is ongoing with more samples and microorganisms quantification.
Methods: Biological materials were collected from the abutment surfaces and implant supported temporary restorations. Bacterial DNA was extracted using sodium hydroxide based chemical protocol and the presence of specific bacteria was evaluated by means of polymerase chain reaction (PCR), using previously designed species specific primers. The temporary crowns on temporary abutments were used for conditioning and contouring the emergence profile on peri-implant soft tissue. Provisionals were made from composite materials and were used for 3-6 months before the insertion of the final restoration.
Results: In the analysed biomaterial from 13 temporary crowns, Fusobacterium spp dominated (in 84.6% samples), followed by Porphyromonas gingivalis (in 61.5% samples). Aggregatibacter actinomycetemcomitans and Tannerella forsythia were found in 38.5% samples and rarely present were Treponema denticola and Prevotella intermedia (in 7.7% samples). In most cases (63.6%), Fusobacterium spp were identified together with Porphyromonas gingivalis. Samples mostly contained 4 or 5 periodontopathogens (together in 69.5% cases), and in 7.7% of cases all examined pathogens were identified.
Conclusions: The results revealed the presence of periodontopathogens on analysed surfaces. Precise identification of different types of periodontopathogens in bacterial colonization of implant abutments and gingival surfaces of the crowns is important for early detection of the infection and consequently for the prevention and proper treatment of periimplant disease. Further research is ongoing with more samples and microorganisms quantification.