IADR Abstract Archives
Evaluation of Apoptosis and Oxidation Mechanisms in Advanced Periodontitis
Objectives: The aim of this study is to evaluate the apoptosis and oxidation mechanisms in systemically healthy Stage III and Stage IV, Grade C periodontitis patients in relation to clinical status. Additionally, correlation between clinical status, oxidative stress and apoptosis markers is examined.
Methods: This study was conducted with the permission of Istanbul University Clinical Studies Local Ethics Committee. Study group consists of 30 systemically healthy individuals diagnosed with Stage III and Stage IV, Grade C periodontitis between ages 18 and 45. Control group consists of 29 systemically and periodontally healthy individuals between ages 18 and 45. Apoptosis and oxidation events were screened by evaluating reactive oxygen metabolites, IL-1β, TNFα, Caspase-3, Caspase-8, Caspase-9, HIF-1α, total antioxidant and total oxidant levels in saliva and gingival crevicular fluid(GCF) samples. Clinical screening was performed by recording Plaque Index (PI), Gingival Index (GI), Probing Pocket Depth (PPD), Clinical Attachment Level (CAL) , Bleeding on Probing (BOP) and tooth mobility.
Kolmogorov-Smirnov test was applied to examine the distribution of the data. Mann-Whitney U test was used to compare clinical parameters and biochemical findings between study and control groups. Pearson Correlation Analysis was performed to examine the correlation between clinical and biochemical variables. All statistical analyzes in the study were performed using SPSS, v.22.0 statistical software. P <0.05 was considered statistically significant.
Results: CAL, PI, GI, PPD, OSI, TNF-α, Luminol-mediated and Lucigenin-mediated Chemiluminescence findings and Caspase-3 level were found to be strongly correlated[u1] . Age, PI, GI, PPD, BOP, CAL, Mobility, TNF-α, Caspase-3, TAOC, TOC, OSI, Luminol-mediated and Lucigenin-mediated Chemiluminescence findings were found statistically significantly different in the periodontitis group compared to the control.
Conclusions: Our findings show that hypoxia is associated with the increase of reactive oxygen molecules, which in turn result in the increase of apoptosis markers. Periodontal status was significantly correlated with oxidation and apoptosis in stage III and IV grade C periodontitis patients.
Methods: This study was conducted with the permission of Istanbul University Clinical Studies Local Ethics Committee. Study group consists of 30 systemically healthy individuals diagnosed with Stage III and Stage IV, Grade C periodontitis between ages 18 and 45. Control group consists of 29 systemically and periodontally healthy individuals between ages 18 and 45. Apoptosis and oxidation events were screened by evaluating reactive oxygen metabolites, IL-1β, TNFα, Caspase-3, Caspase-8, Caspase-9, HIF-1α, total antioxidant and total oxidant levels in saliva and gingival crevicular fluid(GCF) samples. Clinical screening was performed by recording Plaque Index (PI), Gingival Index (GI), Probing Pocket Depth (PPD), Clinical Attachment Level (CAL) , Bleeding on Probing (BOP) and tooth mobility.
Kolmogorov-Smirnov test was applied to examine the distribution of the data. Mann-Whitney U test was used to compare clinical parameters and biochemical findings between study and control groups. Pearson Correlation Analysis was performed to examine the correlation between clinical and biochemical variables. All statistical analyzes in the study were performed using SPSS, v.22.0 statistical software. P <0.05 was considered statistically significant.
Results: CAL, PI, GI, PPD, OSI, TNF-α, Luminol-mediated and Lucigenin-mediated Chemiluminescence findings and Caspase-3 level were found to be strongly correlated[u1] . Age, PI, GI, PPD, BOP, CAL, Mobility, TNF-α, Caspase-3, TAOC, TOC, OSI, Luminol-mediated and Lucigenin-mediated Chemiluminescence findings were found statistically significantly different in the periodontitis group compared to the control.
Conclusions: Our findings show that hypoxia is associated with the increase of reactive oxygen molecules, which in turn result in the increase of apoptosis markers. Periodontal status was significantly correlated with oxidation and apoptosis in stage III and IV grade C periodontitis patients.