IADR Abstract Archives

Biological Responses of Calcium-Silicate-Based Cements on a 3D Pulp Analogue

Objectives: Aim of this study was to evaluate biological responses of two bioactive calcium silicate-based cements (MTA and Biodentine™) on a three-dimensional, tissue-engineered organotypic pulp analogue.
Methods: For the establishment of the pulp analogue, Human Umbilical Vein Endothelial Cells (HUVEC) and Stem Cells from Human Exfoliated Deciduous teeth (SHED) were first co-cultured in 2D conventional culture flasks at a ratio of HUVEC:SHED=3:1. After 24h, the co-cultures were trypsinized and the pellet incorporated into a collagen I/fibrin hydrogel (total protein content 3.5mg/ml), at a concentration of (2.5-6.5)×105cells/hydrogel, depending on the experimental procedure. Biodentine™ and MTA cylindrical specimens (6.5×2mm) were prepared and placed in direct contact with the cell-seeded hydrogels 48h later, whereas hydrogels without specimens served as control. Live/dead staining, MTT assay and Scanning Electron Microscopy (SEM) were used to evaluate cell viability/proliferation and morphology. Real-time PCR to evaluate expression of angiogenic markers (PECAM-1, VEGFa, VEGFR1, VEGFR2, ANGPT-1, Tie-2).
Results: Live/dead staining at day 3 after treatment showed that viable- dominated over non-viable cells (not exceeding 10% for both materials). Cells were evenly distributed inside the hydrogels exposed to the bioactive cements without any differences compared to the control (untreated) cultures. MTT analysis at day 1 and 3 showed that viability/proliferation inside the cell-seeded hydrogels was not influenced by the presence of the bioactive cements. Real-time PCR at day 3 showed upregulation of VEGF and ANGPT-1 in both treated and untreated hydrogels, whereas the respective receptors VEGFR2, VEGFR1 and Tie-2 were downregulated. These alterations were more pronounced in the silica cement-treated groups.
Conclusions: Both calcium silicate-based materials are biocompatible and exert a positive angiogenic effect on the organotypic pulp analogue, validating further application in vital pulp therapy of deciduous teeth.

2021 Continental European and Scandinavian Divisions Meeting (Brussels, Belgium, Hybrid)
Brussels, Belgium, Hybrid
2021
0320
Pulp Biology & Regeneration Research
  • Koutrouli, Anna  ( Aristotle University of Thessaloniki , Thessaloniki , Greece )
  • Arapostathis, Konstantinos  ( Aristotle University of Thessaloniki , Thessaloniki , Greece )
  • Kokoti, Maria  ( Aristotle University of Thessaloniki , Thessaloniki , Greece )
  • Bakopoulou, Athina  ( Aristotle University of Thessaloniki , Thessaloniki , Greece )
  • NONE
    Poster Session ALL VIRTUAL
    Pulp biology & regeneration