IADR Abstract Archives

Gene Expression Profiling of Dental Pulp- and Apical Papilla-Derived Cells

Objectives: The application of both dental pulp stem cells (DPSC) and stem cells from the apical papilla (SCAP) in regenerative endodontic protocols is conceivable. The aim of this study was to identify similarities and differences between DPSC and SCAP regarding their gene expression profiles during induced osteogenic differentiation.
Methods: Primary cells from dental pulp and apical papilla were isolated from an extracted third molar of an 18-year-old patient and analysed for mesenchymal stem cell markers (CD73, CD90, CD105) by flow cytometry. Multi-lineage differentiation was assessed by adipogenic, chondrogenic and osteogenic culture protocols. Transcriptome-wide gene expression of DPSC and SCAP during osteogenic differentiation was profiled by Clariom™ S Human Arrays over 14 days (n = 2). A control was set up by minimum essential medium α supplemented with 10 % fetal bovine serum. Transcriptome Analysis Console 4.0 Software was used for microarray data analysis and genes were ranked by the empirical Bayes method. Gene regulation (fold-change > 10; P-value < 0.01) was evaluated in the context of culture parameters (timepoint, type of medium, cell type) and group selections were made to identify similarities and differences.
Results: A high proportion of DPSC and SCAP expressed mesenchymal stem cell markers and both cell types were able to enter all the lineages investigated. Transcriptome analysis revealed the greatest gene regulation over the differentiation period, whereas medium and cell type were only secondary variables. During osteogenic differentiation, fewer genes were regulated for both cell types compared to the undifferentiated control (10 % FBS). However, 13 common key genes were identified during induced osteogenic differentiation for DPSC and SCAP: GPX3, PIP, IGFBP2, SAA2/SAA2-SAA4/SAA4, SAA1, GPM6B, FAM107A, LEPR/LEPROT, PAPPA, ABCA6, VWA5A, PDE1A, ID3.
Conclusions: DPSC and SCAP appear to share similarities not only in terms of origin, but also in terms of regulation of gene expression during the process of cell differentiation.

2021 Continental European and Scandinavian Divisions Meeting (Brussels, Belgium, Hybrid)
Brussels, Belgium, Hybrid
2021
0319
Pulp Biology & Regeneration Research
  • Smeda, Martyna  ( University Hospital Regensburg , Regensburg , Germany )
  • Galler, Kerstin  ( University Hospital Regensburg , Regensburg , Germany )
  • Wölflick, Melanie  ( University Hospital Regensburg , Regensburg , Germany )
  • Rosendahl, Andreas  ( University Hospital Regensburg , Regensburg , Germany )
  • Bucchi, Cristina  ( Universidad de La Frontera , Temuco , Chile )
  • Buchalla, Wolfgang  ( University Hospital Regensburg , Regensburg , Germany )
  • Widbiller, Matthias  ( University Hospital Regensburg , Regensburg , Germany )
  • NONE
    Poster Session ALL VIRTUAL
    Pulp biology & regeneration