IADR Abstract Archives
Osteoconductivity of BSA Incorporated Amorphous and Crystalline Calcium Phosphate Coating
Objectives: An early osteointegration facilitates early loading of orthodontic mini-screws and reduces risk of failure. Biomimetic calcium phosphate (CaP) coating is a well-established surface-modification method of metallic implants to promote osteogenic activity and carry various bioactive agents for further functionalization. And it has been shown that bovine serum albumin (BSA) may promote mineral deposition because they contain structures, such as -OH, -NH, and -SH, with strong affinities for inorganic cations and anions. In this study, we hypothesized that biomimetic amorphous or crystalline CaP coating with BSA facilitated an earlier osteointegration of titanium mini-implants.
Methods: Biomimetic amorphous and crystalline coatings with or without BSA incorporation were prepared on the mini-pins, and both naked and with adsorbed BSA were used as control groups. Enzyme linked immunosorbent assay (ELISA) was utilized to identify the loading and release of BSA. The mini-pins were implanted in the rats tibial for in vivo evaluation of the course of osseointegration. 3 days, 1 week, 2 weeks and 4 weeks post-surgery, the samples were retrieved and subjected into histological and histomorphormetrical analysis to determine bone to implant contact (BIC).
Results: The rate of BSA release from amorphous and crystalline coatings in vitro during the initial 3 days was 81% and 23% respectively, and by the end of the monitoring period (day 35), 96% and 52% of the incorporated BSA had been released. A significant increase of BIC occurred on the 14th day for the mini-pins without any coating and those with amorphous coatings, while the BIC increase occurred already on the 7th day for the mini-pins with crystalline coatings compared to the BIC on the 3rd day. The incorporation of BSA postponed significantly the increase of BIC to a later time point of 35 days.
Conclusions: We confirmed that biomimetic crystalline coatings can lead a slow release of the protein and facilitate an earlier increase of bone to implants contact.
Methods: Biomimetic amorphous and crystalline coatings with or without BSA incorporation were prepared on the mini-pins, and both naked and with adsorbed BSA were used as control groups. Enzyme linked immunosorbent assay (ELISA) was utilized to identify the loading and release of BSA. The mini-pins were implanted in the rats tibial for in vivo evaluation of the course of osseointegration. 3 days, 1 week, 2 weeks and 4 weeks post-surgery, the samples were retrieved and subjected into histological and histomorphormetrical analysis to determine bone to implant contact (BIC).
Results: The rate of BSA release from amorphous and crystalline coatings in vitro during the initial 3 days was 81% and 23% respectively, and by the end of the monitoring period (day 35), 96% and 52% of the incorporated BSA had been released. A significant increase of BIC occurred on the 14th day for the mini-pins without any coating and those with amorphous coatings, while the BIC increase occurred already on the 7th day for the mini-pins with crystalline coatings compared to the BIC on the 3rd day. The incorporation of BSA postponed significantly the increase of BIC to a later time point of 35 days.
Conclusions: We confirmed that biomimetic crystalline coatings can lead a slow release of the protein and facilitate an earlier increase of bone to implants contact.