IADR Abstract Archives

Tension Effect on Human Periodontal Ligament Cells: a Systematic Review

Objectives: To identify all studies in orthodontic tooth movement using an in vitro loading tension model applied to human periodontal ligament cells. Secondly, to summarize their findings regarding cell source, force parameters (apparatus, magnitude, frequency, duration) and gene expression and to identify the most significant signaling pathways.
Methods: We followed the PRISMA guideline for systematic reviews. To identify all related studies, an appropriate PubMed search strategy was developed. Selection of the studies was done according to predetermined eligibility criteria. Data of interest (cell source, force and expression related) were extracted into structured tables. Risk of bias in reporting and methodology was assessed with guidelines for in vitro studies. Regulated gene sets were analyzed using STRING-DB and GeneAnalytics.
Results: From initially 5,331 identified publications, 137 studies were included with relevant information being extracted and unified. Methodological quality including confounding variables, sample size determination, statistical analysis and optimal time window and reporting quality of justification for the model were identified as the most obvious high risk of bias. Tension application was done either dynamically (103/137) or statically (30/137). Dynamic tension was most frequently applied by commercial systems with magnitude of 10% or 12%. The most common frequencies were 0.1Hz and 0.5Hz for equibiaxial strain and 0.5Hz for uniaxial strain, both for up to 72h. Static tension was applied mostly using flexible-bottom culture dishes (2.5%) or plates (10%) for up to 24h. The most common genes or products were related to osteogenesis (RUNX2, ALPP, BGLAP, TNFRSF11B, COL1A1, SP7, SPP1), osteoclastogenesis and inflammation (IL1B, PTGS2, TNFSF11, PGE2, TNF, CXCL8, IL6) and apoptosis (CASP1, CASP3, CASP5).
Conclusions: We identified the most common force parameters and related gene expressions. Using the pathways and force parameters identified, tension models can be established to investigate the effects of different magnitudes and durations on related biological regulations.

2021 Continental European and Scandinavian Divisions Meeting (Brussels, Belgium, Hybrid)
Brussels, Belgium, Hybrid
2021
0136
Orthodontics Research
  • Sun, Changyun  ( University Hospital, LMU Munich , Munich , Germany )
  • Janjic Rankovic, Mila  ( University Hospital, LMU Munich , Munich , Germany )
  • Folwaczny, Matthias  ( University Hospital, LMU Munich , Munich , Germany )
  • Otto, Sven  ( Martin-Luther-University Halle-Wittenberg , Halle (Saale) , Germany )
  • Wichelhaus, Andrea  ( University Hospital, LMU Munich , Munich , Germany )
  • Baumert, Uwe  ( University Hospital, LMU Munich , Munich , Germany )
  • NONE
    Changyun Sun was supported by a grant from the China Scholarship Council (CSC File No 201809370043).
    Oral Session VIRTUAL
    Orthodontics