IADR Abstract Archives
Bone Regeneration in Apical Periodontitis
Objectives: It has long been discussed that the healing rate of asymptomatic apical periodontitis (AAP) is contingent on the treatment protocol in terms of number of visits, preparation techniques and medicaments. However, bone healing is an intricate multifaceted process of cellular proliferation. The aim of this study was to examine a potential link between patients’ mitogen-induced inflammatory mediator release and the rate of bone healing following non-surgical endodontic treatment of AAP
Methods: Patients (n=20) diagnosed with AAP were enrolled in the study and underwent non-surgical endodontic treatment. Small field-of-view cone beam computed tomography was used to assess the lesion volume prior to and 10 months following treatment. Pre-treatment peripheral blood samples were collected from the patients and peripheral blood mononuclear cells were isolated and cultured in two different media: RPMI 1640 and RPMI 1640 + PHA. The supernatant was collected at the 24th and 48th hour and the levels of IL-1β and PGE2 were assessed by ELISA. Individual mitogen-induced reactivity (MIR) was calculated for each molecule. Logistic regression, extra tree classifier, ANOVA and heatmap methods were used to assess the association between the levels of MIR of each molecule and the rate of healing.
Results: The whole set of used algorithms outlined MIR PGE2/24h , MIR PGE2/48 and MIR IL-1β/48 as strong predictors of the rate of bone healing.
Conclusions: A link between the host’s mitogen-induced reactivity and the rate of bone regeneration was observed. Further studies can shed light on the underlying pathways of the observed phenomenon and translate these finding into the clinical practice.
Methods: Patients (n=20) diagnosed with AAP were enrolled in the study and underwent non-surgical endodontic treatment. Small field-of-view cone beam computed tomography was used to assess the lesion volume prior to and 10 months following treatment. Pre-treatment peripheral blood samples were collected from the patients and peripheral blood mononuclear cells were isolated and cultured in two different media: RPMI 1640 and RPMI 1640 + PHA. The supernatant was collected at the 24th and 48th hour and the levels of IL-1β and PGE2 were assessed by ELISA. Individual mitogen-induced reactivity (MIR) was calculated for each molecule. Logistic regression, extra tree classifier, ANOVA and heatmap methods were used to assess the association between the levels of MIR of each molecule and the rate of healing.
Results: The whole set of used algorithms outlined MIR PGE2/24h , MIR PGE2/48 and MIR IL-1β/48 as strong predictors of the rate of bone healing.
Conclusions: A link between the host’s mitogen-induced reactivity and the rate of bone regeneration was observed. Further studies can shed light on the underlying pathways of the observed phenomenon and translate these finding into the clinical practice.