IADR Abstract Archives
Changes in the Composition of Radicular Dentin Using Passive Ultrasonic Irrigation
Objectives: OBJETIVE: To analyze the composition of radicular dentin with ATR-FTIR spectroscopy after immersion in different NaOCl concentrations with application or not of passive ultrasonic irrigation (PUI).
Methods: METHODS: Sixteen human single-root teeth were sectioned and one slice from each radicular third was selected. Specimens were randomly distributed into four groups: (1) 2.25 % NaOCl for 1, 5 and 20 min; (2) 2.25 % NaOCl for 1, 5 and 20 min with PUI; (3) 5.25 % NaOCl for 1, 5 and 20 min; (4) 5.25 % NaOCl for 1, 5 and 20 min with PUI. Carbonate/mineral, Amide I/mineral and Amide III/CH2 ratios were determined using ATR-FTIR spectroscopy for each specimen before and after treatment. Data were analyzed by Kruskal-Wallis, Mann-Whitney U, and repeated measures ANOVA tests (p<0.05).
Results: RESULTS: The 2.25 % NaOCl solution did not modify carbonate/mineral ratio, although significantly decreased Amide I/mineral and Amide III/CH2 ratios. The application of PUI significantly decreased the carbonate/mineral ratio in the coronal third after 5 min of immersion in NaOCl, and the Amide I/mineral and Amide III/ ratios, after 1 min and 20 min, respectively. 5.25 % NaOCl significantly decreased carbonate/mineral, Amide I/mineral, and Amide III/CH2 ratios up to 5 minutes after treatment with a higher decrease with PUI application. Root third did not affect composition of radicular dentin regardless of the irrigation treatment tested
Conclusions:
CONCLUSION: The organic and inorganic composition of root dentin was affected by NaOCl concentration and the application of PUI. The most pronounced change was detected after 5.25% irrigation with PUI. The activation of NaOCl significantly modified carbonate/mineral, Amide I/mineral, and Amide III/CH2 ratios for both NaOCl concentrations even after application for one minute
Methods: METHODS: Sixteen human single-root teeth were sectioned and one slice from each radicular third was selected. Specimens were randomly distributed into four groups: (1) 2.25 % NaOCl for 1, 5 and 20 min; (2) 2.25 % NaOCl for 1, 5 and 20 min with PUI; (3) 5.25 % NaOCl for 1, 5 and 20 min; (4) 5.25 % NaOCl for 1, 5 and 20 min with PUI. Carbonate/mineral, Amide I/mineral and Amide III/CH2 ratios were determined using ATR-FTIR spectroscopy for each specimen before and after treatment. Data were analyzed by Kruskal-Wallis, Mann-Whitney U, and repeated measures ANOVA tests (p<0.05).
Results: RESULTS: The 2.25 % NaOCl solution did not modify carbonate/mineral ratio, although significantly decreased Amide I/mineral and Amide III/CH2 ratios. The application of PUI significantly decreased the carbonate/mineral ratio in the coronal third after 5 min of immersion in NaOCl, and the Amide I/mineral and Amide III/ ratios, after 1 min and 20 min, respectively. 5.25 % NaOCl significantly decreased carbonate/mineral, Amide I/mineral, and Amide III/CH2 ratios up to 5 minutes after treatment with a higher decrease with PUI application. Root third did not affect composition of radicular dentin regardless of the irrigation treatment tested
Conclusions:
CONCLUSION: The organic and inorganic composition of root dentin was affected by NaOCl concentration and the application of PUI. The most pronounced change was detected after 5.25% irrigation with PUI. The activation of NaOCl significantly modified carbonate/mineral, Amide I/mineral, and Amide III/CH2 ratios for both NaOCl concentrations even after application for one minute