Effect of Crosslinkers on Ultimate Tensile Strength of Demineralized Dentin

Objectives: Biomodification of dentin by collagen crosslinkers improves durability of collagen matrices in hybrid layer. Aim of this study is to evaluate the effect of five different crosslinkers on dentin ultimate tensile strength (UTS) and to detect total phenolic content (TBC) of crosslinkers.
Methods: Dentin beams (1 mm × 2 mm × 6 mm) were demineralized in 10% H₃PO₄ for 24 h. and, beams were divided into 17 groups (n = 10/group). Five different crosslinkers; 1% and 5% glutaraldehyde, 0,01% riboflavin-5-monophospate/UVA, 10% sumac berry extract, 1% and 5% grape seed extract, and curcumin were used. Demineralized dentin beams were pretreated with respective crosslinkers for 1 or 5 min and control (no pretreatment). The changes in tensile strength of dentin was evaluated by UTS using a universal testing machine. The total polyphenol content of the extracts was determined (as gallic acid equivalents) using Folin- Ciocalteau assay. Data were analyzed by two way- and one way- ANOVA respectively.



Results: The result of the study showed that crosslinker treated groups showed significantly higher UTS range from 14,15±2,74 and 9,92±2,82 compared to untreated control (6,7±2) . Both concentration and treatment time of crosslinker significantly increased UTS (p<0.05). Grape seed extract (83,2±7,4 and 349±33,2) and sumac berry extract (73,3±3,3) showed significant high total phenolic content compared to other crosslinkers (p<0.05).
Conclusions: Collagen crosslinkers induced a significant increase in UTS and bioactivities of crosslinkers are source dependent, resulting from a combination of concentration and specific chemical constitution of the complex mixtures.