IADR Abstract Archives
Regional Effect of Epinephrine on the Microcirculation of Human Gingiva
Objectives: Spreading vasoconstriction is a known phenomenon which is described in various tissues as a locally evoked vasoconstriction which could elicit remote vasoconstriction at distant areas. Epinephrine is widely used vasoconstrictor in dentistry, but the spreading vasoconstriction has not been demonstrated yet in gingiva.
The aim of this study was to investigate the local and remote effect of epinephrine in the attached gingiva.
Methods: Gingival blood flow was measured by Laser Speckle Contrast Imager in 15 healthy volunteers. In group A two wells were fabricated from orthodontic ligatures for the solutions and placed 2 mm apically from the free gingival margin at midbuccal line of 12 and 21 teeth. Blood flow was measured in the wells and apical, coronal, distal, mesial directions around the wells. In group B the wells were made on the surface of the corresponding teeth including the gingival sulcus and four measurement regions were from the gingival margin reaching to the mucogingival line close to each other. After the baseline recording either 1 mg/ml epinephrine solution (test site) and physiological saline (control site) were applied into the wells and further 15 minutes recording was done.
Results: : In group A the gingival blood flow did not changed in any regions. In group B the blood flow decreased at the test site immediately after application of epinephrine and remained low for 15 minutes in all regions. The decrease was the largest close to sulcus which degree became lower to the apical direction.
Conclusions: Attached gingiva seems to be impermeable for epinephrine thus epinephrine cannot evoke any vasoconstriction on the keratinized gingiva. Whereas application at the gingival sulcus could evoke strong, long-lasting vasoconstriction up to the mucogingival junction suggesting the permeability of sulcus and presence of spreading vasoconstriction in the human gingiva.
Supported by Hungarian OTKA K112364 and KFI_16-1-2017-0409.
The aim of this study was to investigate the local and remote effect of epinephrine in the attached gingiva.
Methods: Gingival blood flow was measured by Laser Speckle Contrast Imager in 15 healthy volunteers. In group A two wells were fabricated from orthodontic ligatures for the solutions and placed 2 mm apically from the free gingival margin at midbuccal line of 12 and 21 teeth. Blood flow was measured in the wells and apical, coronal, distal, mesial directions around the wells. In group B the wells were made on the surface of the corresponding teeth including the gingival sulcus and four measurement regions were from the gingival margin reaching to the mucogingival line close to each other. After the baseline recording either 1 mg/ml epinephrine solution (test site) and physiological saline (control site) were applied into the wells and further 15 minutes recording was done.
Results: : In group A the gingival blood flow did not changed in any regions. In group B the blood flow decreased at the test site immediately after application of epinephrine and remained low for 15 minutes in all regions. The decrease was the largest close to sulcus which degree became lower to the apical direction.
Conclusions: Attached gingiva seems to be impermeable for epinephrine thus epinephrine cannot evoke any vasoconstriction on the keratinized gingiva. Whereas application at the gingival sulcus could evoke strong, long-lasting vasoconstriction up to the mucogingival junction suggesting the permeability of sulcus and presence of spreading vasoconstriction in the human gingiva.
Supported by Hungarian OTKA K112364 and KFI_16-1-2017-0409.