IADR Abstract Archives
In-vitro Investigation of the Effectiveness of Different Endodontic Irrigation Solutions Used Alone or Combined with other Irrigants against Enterococcus Faecalis
Objectives: The purpose of this study is to assess the antibacterial activity of both separate and combined uses of 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), 17% ethylenediaminetetraacetic acid (EDTA), 3% hydrogen peroxide (H2O2), MTAD, SmearClear (SC) and 13.8% chlorine dioxide (ClO2) solutions against Enterococcus faecalis
Methods: 280 single-rooted permanent human lower premolars were randomly grouped into 26 test and 2 control groups; the test groups were incubated for 24 hours with E. faecalis (ATCC 29212). The tested solutions and combinations were as follows: NaOCl, CHX, ClO2, MTAD, SC, EDTA, H2O2, NaOCl+CHX, NaOCl+MTAD, SC+NaOCl, EDTA+NaOCl, H2O2+NaOCl, ClO2+CHX, CHX+MTAD, SC+CHX, EDTA+CHX, H2O2+CHX, ClO2+MTAD, SC+ClO2, EDTA+ClO2, H2O2+ClO2, SC+MTAD, EDTA+MTAD, H2O2+MTAD, SC+H2O2 and EDTA+H2O2. For spectrophotometric analyses, the samples were transferred to an ELISA plate, and optic density values were recorded at 0, 6, 12, 18, 24, 30, 36, 42 and 48 hours for each sample. The data were statistically analysed using the Kruskal–Wallis and Mann–Whitney U tests. In addition, bacterial growth curves were created for each solution and its combinations.
Results: In E. faecalis elimination, the antibacterial activity of H2O2, H2O2+NaOCl and EDTA were not found to have as much activity as the ClO2, SC, MTAD and CHX combinations. EDTA did not show any antibacterial activity either alone or in any combination, except EDTA+CHX and EDTA+MTAD. MTAD showed antibacterial activity both alone and in all combinations. SC showed antibacterial activity in all combinations, except SC+NaOCl. CHX and all its combinations showed significant antibacterial activity against E. faecalis.
Conclusions: The H2O2, EDTA and SC combinations of NaOCl were not found to be effective in E. faecalis elimination. ClO2 and all its combinations were found to be significantly effective against E. faecalis.
Methods: 280 single-rooted permanent human lower premolars were randomly grouped into 26 test and 2 control groups; the test groups were incubated for 24 hours with E. faecalis (ATCC 29212). The tested solutions and combinations were as follows: NaOCl, CHX, ClO2, MTAD, SC, EDTA, H2O2, NaOCl+CHX, NaOCl+MTAD, SC+NaOCl, EDTA+NaOCl, H2O2+NaOCl, ClO2+CHX, CHX+MTAD, SC+CHX, EDTA+CHX, H2O2+CHX, ClO2+MTAD, SC+ClO2, EDTA+ClO2, H2O2+ClO2, SC+MTAD, EDTA+MTAD, H2O2+MTAD, SC+H2O2 and EDTA+H2O2. For spectrophotometric analyses, the samples were transferred to an ELISA plate, and optic density values were recorded at 0, 6, 12, 18, 24, 30, 36, 42 and 48 hours for each sample. The data were statistically analysed using the Kruskal–Wallis and Mann–Whitney U tests. In addition, bacterial growth curves were created for each solution and its combinations.
Results: In E. faecalis elimination, the antibacterial activity of H2O2, H2O2+NaOCl and EDTA were not found to have as much activity as the ClO2, SC, MTAD and CHX combinations. EDTA did not show any antibacterial activity either alone or in any combination, except EDTA+CHX and EDTA+MTAD. MTAD showed antibacterial activity both alone and in all combinations. SC showed antibacterial activity in all combinations, except SC+NaOCl. CHX and all its combinations showed significant antibacterial activity against E. faecalis.
Conclusions: The H2O2, EDTA and SC combinations of NaOCl were not found to be effective in E. faecalis elimination. ClO2 and all its combinations were found to be significantly effective against E. faecalis.
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