IADR Abstract Archives
Antibacterial Effects of Polyethylenimine, Nanosilver-Polyethylenimine Complex on Selected Oral Microorganisms
Objectives: Polyethylenimine (PEI) is a highly branching, polycationic polymer with antibacterial property because of its large number, positively charged, terminally positioned amino groups. The aim of this study was to test the antimicrobial effects of PEI and nanosilver-PEI complex (Ag-PEI) on selected oral test microorganisms.
Methods: Minimum inhibitory concentrations (MIC) of agents were determined by broth microdilution assay. Well diffusion technique and disk diffusion test were also applied to measure the 24 hours inhibitory zones of different concentrations of agents on Mueller-Hinton and blood agar for Enterococcus faecalis (ATCC29212), with additional 4% of glucose for Candida albicans (ATCC66027), Mitis-Salivarius and blood agar for Streptococcus mutans (ATCC35668) in 37C° thermostat and 5% CO2.
Results: MIC values for PEI and Ag-PEI were at E. faecalis: 0.125% and 0.2%, C. albicans: 0.05% for both agents, and S. mutans: 0.125% and 0.2%, respectively. Both drugs increased in a similar way the diameters of the inhibitory zone in a concentration-dependent manner at all tested microorganisms. However, out of the three investigated microorganisms, the agar plates with C. albicans formed consequently the highest inhibitory zone at the same drug concentrations.
Conclusions: PEI and Ag-PEI were found to inhibit the growth of microorganisms by similar extent and the fungi seemed more sensitive than bacteria. Different dental materials such as dental acrylic, composite resins or endodontic sealants incorporated or coated with PEI or Ag-PEI may prevent and reduce the diseases causing bacterial and fungal growths and infections. Supported by Hungarian NKFIH_OTKA_K112364 and KFI_16-1-2017-0409.
Methods: Minimum inhibitory concentrations (MIC) of agents were determined by broth microdilution assay. Well diffusion technique and disk diffusion test were also applied to measure the 24 hours inhibitory zones of different concentrations of agents on Mueller-Hinton and blood agar for Enterococcus faecalis (ATCC29212), with additional 4% of glucose for Candida albicans (ATCC66027), Mitis-Salivarius and blood agar for Streptococcus mutans (ATCC35668) in 37C° thermostat and 5% CO2.
Results: MIC values for PEI and Ag-PEI were at E. faecalis: 0.125% and 0.2%, C. albicans: 0.05% for both agents, and S. mutans: 0.125% and 0.2%, respectively. Both drugs increased in a similar way the diameters of the inhibitory zone in a concentration-dependent manner at all tested microorganisms. However, out of the three investigated microorganisms, the agar plates with C. albicans formed consequently the highest inhibitory zone at the same drug concentrations.
Conclusions: PEI and Ag-PEI were found to inhibit the growth of microorganisms by similar extent and the fungi seemed more sensitive than bacteria. Different dental materials such as dental acrylic, composite resins or endodontic sealants incorporated or coated with PEI or Ag-PEI may prevent and reduce the diseases causing bacterial and fungal growths and infections. Supported by Hungarian NKFIH_OTKA_K112364 and KFI_16-1-2017-0409.