IADR Abstract Archives
Infiltration of White Spot Lesions with a New Hybrid-Glass-Based Material
Objectives: The objective of this study was to compare the effectiveness of Icon (DMG, Hamburg, Germany) and two experimental, hybrid-glass-based infiltrating materials (Hybrid Glass, Wroclaw, Poland), in preventing the progression of artificial white spot lesions (WSLs), in vitro.
Methods: Artificial WSLs were formed on 3mm-wide middle part of disk-shaped bovine enamel specimens (n=68; 8mm diameter), while the outer parts were protected and served as a reference. Samples were divided into four groups according to the treatment of WSLs: 1) no-treatment control (NTC), 2) infiltration with Icon (I), 3) infiltration with experimental hybrid-glass material (EXP), and 4) infiltration with experimental hybrid-glass material containing hydroxyapatite nanoparticles (1%) (EXP-HAp). Half of the specimens from each group were subjected to a cariogenic challenge using pH-cycling protocol. Briefly, during 5-day period specimens were incubated (37°C) alternately in demineralization (4h/day, pH=4.6) and remineralization solutions (20h/day, pH=7.2). Another half of the specimens was incubated (37°C) in distilled water and served as a control. Finally, enamel softening was assessed by measuring surface micro-hardness, enamel surface loss was determined by measuring surface roughness and profilometry, and surface morphology was analyzed with SEM. One-Way ANOVA and post-hoc LSD or Games-Howell tests were used for data comparison (p<0.05).
Results: In all groups (NTC, I and EXP-HAp), except in EXP group, surface micro-hardness decreased significantly after pH cycling. In addition, micro-hardness in EXP group was significantly higher than in other pH-cycled groups (p<0.05). Also, surface roughness increased considerably while profilometry showed a significant tissue loss after pH cycling in all groups except in EXP group. This tissue loss and increase in roughness in NTC, I and EXP-HAp groups was also observed with SEM.
Conclusions: An experimental hybrid-glass material without HAp-nanoparticles seems to be able to completely arrest the progression of WSLs, unlike its version with HAp-nanoparticles and resin-based infiltrant Icon.
Methods: Artificial WSLs were formed on 3mm-wide middle part of disk-shaped bovine enamel specimens (n=68; 8mm diameter), while the outer parts were protected and served as a reference. Samples were divided into four groups according to the treatment of WSLs: 1) no-treatment control (NTC), 2) infiltration with Icon (I), 3) infiltration with experimental hybrid-glass material (EXP), and 4) infiltration with experimental hybrid-glass material containing hydroxyapatite nanoparticles (1%) (EXP-HAp). Half of the specimens from each group were subjected to a cariogenic challenge using pH-cycling protocol. Briefly, during 5-day period specimens were incubated (37°C) alternately in demineralization (4h/day, pH=4.6) and remineralization solutions (20h/day, pH=7.2). Another half of the specimens was incubated (37°C) in distilled water and served as a control. Finally, enamel softening was assessed by measuring surface micro-hardness, enamel surface loss was determined by measuring surface roughness and profilometry, and surface morphology was analyzed with SEM. One-Way ANOVA and post-hoc LSD or Games-Howell tests were used for data comparison (p<0.05).
Results: In all groups (NTC, I and EXP-HAp), except in EXP group, surface micro-hardness decreased significantly after pH cycling. In addition, micro-hardness in EXP group was significantly higher than in other pH-cycled groups (p<0.05). Also, surface roughness increased considerably while profilometry showed a significant tissue loss after pH cycling in all groups except in EXP group. This tissue loss and increase in roughness in NTC, I and EXP-HAp groups was also observed with SEM.
Conclusions: An experimental hybrid-glass material without HAp-nanoparticles seems to be able to completely arrest the progression of WSLs, unlike its version with HAp-nanoparticles and resin-based infiltrant Icon.