IADR Abstract Archives
Cytoprotective Effects of Geranylgeraniol on Alendronate-induced MC3T3 Cell Death
Objectives: To investigate the effects of GGOH on MC3T3 cell viability and cell cycle under ALN treatment.
Methods: MC3T3 cells were incubated with different doses of ALN (0-50 µM) and GGOH (0-50 µM). The viability and apoptosis of the MC3T3 treated with ALN and GGOH for 3 d were determined by MTT and annexin/PI staining assays, respectively. Cells stained with PI were used to determine cell cycle phase. In addition, cells were stained with rhodamine pholloidin to investigate cell cytoskeleton.
Results: ALN reduced cell viability of MC3T3 in a dose dependent manner. GGOH at 50 µM partially inhibited the cytotoxic effects of 10 and 50 µM ALN. ALN at 10 µM increased the percentages of cell apoptosis and necrosis and arrested cells in G2M phase. Incubation with 50 µM GGOH partially reduced late cell apoptosis and significantly rescued cell cycle arrest in G2M. Furthermore, ALN altered MC3T3 morphology and decreased cell area as well as actin stress fiber density. GGOH significantly abolished the effect of ALN on cell area and actin stress fiber density.
Conclusions: GGOH partially reversed negative biological effects of ALN on MC3T3 cell viability, cell cycle, morphology and cytoskeleton.
Methods: MC3T3 cells were incubated with different doses of ALN (0-50 µM) and GGOH (0-50 µM). The viability and apoptosis of the MC3T3 treated with ALN and GGOH for 3 d were determined by MTT and annexin/PI staining assays, respectively. Cells stained with PI were used to determine cell cycle phase. In addition, cells were stained with rhodamine pholloidin to investigate cell cytoskeleton.
Results: ALN reduced cell viability of MC3T3 in a dose dependent manner. GGOH at 50 µM partially inhibited the cytotoxic effects of 10 and 50 µM ALN. ALN at 10 µM increased the percentages of cell apoptosis and necrosis and arrested cells in G2M phase. Incubation with 50 µM GGOH partially reduced late cell apoptosis and significantly rescued cell cycle arrest in G2M. Furthermore, ALN altered MC3T3 morphology and decreased cell area as well as actin stress fiber density. GGOH significantly abolished the effect of ALN on cell area and actin stress fiber density.
Conclusions: GGOH partially reversed negative biological effects of ALN on MC3T3 cell viability, cell cycle, morphology and cytoskeleton.