Microbiological Characterization of Different Stages and Grades of Periodontitis
Objectives: To determine to what extent microbiological profiles can distinguish among different stages and grades of the new classification of periodontitis. Methods: Thirty-three subgingival samples, coming from 11 patients without periodontitis, 11 subjects with periodontitis stages 1 and 2, and 11 patients with periodontitis stages 3 and 4, were analyzed by microbiological culture. Grades were given using indirect estimation of progression (radiographic bone loss/age): slow (A), moderate (B) and rapid progression (C). The microbiological study included total anaerobic counts, and frequency of detection, counts and proportions of target species. Association measures, chi-square test with Bonferroni correction and non-parametric methods (Kruskal-Wallis and Jonckheere-Terpstra tests) were used for the statistical analysis. Results: The frequency of detection of the main periodontal pathogens isolated were: Porphyromonas gingivalis (non-periodontitis, 64%; stages 1-2, 82%; stages 3-4, 91%), Prevotella intermedia (64%; 91%; 100%), Tannerella forsythia (9%; 27%; 54%), Parvimonas micra (18%; 0%; 0%) and Fusobacterium nucleatum (91%; 91%; 82%). Total counts showed statistically significant differences among groups (p=0.019), and grades (p=0.047). There were statistically significant concordant trends between total counts and stages (p=0.005) and grades (p=0.015); and between counts of P. intermedia and T. forsythia and periodontal stages (p=0.029 and p=0.028, respectively). Counts and proportions of Eikenella corrodens and Capnocytophaga spp. showed a statistically significant trend for increasing extension of periodontitis. The odds ratio (OR) for the detection of T. forsythia in periodontitis stages 3 and 4 was greater than in non-periodontitis group (OR= 12; 95% confidence interval >1). Conclusions: Distinctive profiles of subgingival microbiota could be discerned among stages and grades, since total counts and counts of P. gingivalis and T. forsythia have shown a significant increase as periodontal stages aggravate; and total counts have also shown a significant trend to increase as the rate of progression worsens from slow (A) to rapid (C).
Division:Continental European and Scandinavian Divisions Meeting
Meeting:2019 Continental European and Scandinavian Divisions Meeting (Madrid, Spain) Location:Madrid, Spain
Year: 2019 Final Presentation ID:0020 Abstract Category|Abstract Category(s):Periodontal Research-Diagnosis/Epidemiology
Authors
Iniesta, Margarita
( Complutense University of Madrid
, Madrid
, Spain
)
Fernández-baca, Ignacio
( University Complutense
, Madrid
, Spain
)
O'connor, Ana
( University Complutense
, Madrid
, Spain
)
Sanz, Mariano
( University Complutense
, Madrid
, Spain
)
Herrera, David
( University Complutense
, Madrid
, Spain
)
Financial Interest Disclosure: NONE
Support Funding Agency/Grant Number: Cátedra Extraordinaria Dentaid de Investigación en Periodoncia
Total counts and counts of target species according to the periodontal condition
Mean ranks
K-W
J-T
Counts
n
Health and gingivitis (n=11)
Periodontitis stages 1-2 (n=11)
Periodontitis stages 3-4 (n=11)
p
p
Total
33
12.55
14.91
23.55
0.019*
0.005*
Porphyromonas gingivalis
33
14.95
14.05
22.00
0.105
0.065
Prevotella intermedia
33
13.45
15.50
22.05
0.092
0.029*
Tannerella intermedia
33
13.55
16.50
20.95
0.084
0.028*
Parvimonas micra
33
19.00
16.00
16.00
0.127
0.079
Fusobacterium nucleatum
33
16.64
15.91
18.45
0.816
0.631
Campylobacter rectus
33
16.36
15.00
19.64
0.125
0.171
Eikenella corrodens
33
16.41
16.41
18.18
0.681
0.448
Capnocytophaga spp.
33
16.36
15.00
19.64
0.125
0.171
n: sample size; K-W: Kruskal-Wallis test; J-T: Jonckheere-Terpstra test; *: p<0.05; spp.: species.
Total counts and counts of target species according to the progression of periodontitis