IADR Abstract Archives
Microbiological Characterization of Different Stages and Grades of Periodontitis
Objectives: To determine to what extent microbiological profiles can distinguish among different stages and grades of the new classification of periodontitis.
Methods: Thirty-three subgingival samples, coming from 11 patients without periodontitis, 11 subjects with periodontitis stages 1 and 2, and 11 patients with periodontitis stages 3 and 4, were analyzed by microbiological culture. Grades were given using indirect estimation of progression (radiographic bone loss/age): slow (A), moderate (B) and rapid progression (C). The microbiological study included total anaerobic counts, and frequency of detection, counts and proportions of target species. Association measures, chi-square test with Bonferroni correction and non-parametric methods (Kruskal-Wallis and Jonckheere-Terpstra tests) were used for the statistical analysis.
Results: The frequency of detection of the main periodontal pathogens isolated were: Porphyromonas gingivalis (non-periodontitis, 64%; stages 1-2, 82%; stages 3-4, 91%), Prevotella intermedia (64%; 91%; 100%), Tannerella forsythia (9%; 27%; 54%), Parvimonas micra (18%; 0%; 0%) and Fusobacterium nucleatum (91%; 91%; 82%). Total counts showed statistically significant differences among groups (p=0.019), and grades (p=0.047). There were statistically significant concordant trends between total counts and stages (p=0.005) and grades (p=0.015); and between counts of P. intermedia and T. forsythia and periodontal stages (p=0.029 and p=0.028, respectively). Counts and proportions of Eikenella corrodens and Capnocytophaga spp. showed a statistically significant trend for increasing extension of periodontitis. The odds ratio (OR) for the detection of T. forsythia in periodontitis stages 3 and 4 was greater than in non-periodontitis group (OR= 12; 95% confidence interval >1).
Conclusions: Distinctive profiles of subgingival microbiota could be discerned among stages and grades, since total counts and counts of P. gingivalis and T. forsythia have shown a significant increase as periodontal stages aggravate; and total counts have also shown a significant trend to increase as the rate of progression worsens from slow (A) to rapid (C).
Methods: Thirty-three subgingival samples, coming from 11 patients without periodontitis, 11 subjects with periodontitis stages 1 and 2, and 11 patients with periodontitis stages 3 and 4, were analyzed by microbiological culture. Grades were given using indirect estimation of progression (radiographic bone loss/age): slow (A), moderate (B) and rapid progression (C). The microbiological study included total anaerobic counts, and frequency of detection, counts and proportions of target species. Association measures, chi-square test with Bonferroni correction and non-parametric methods (Kruskal-Wallis and Jonckheere-Terpstra tests) were used for the statistical analysis.
Results: The frequency of detection of the main periodontal pathogens isolated were: Porphyromonas gingivalis (non-periodontitis, 64%; stages 1-2, 82%; stages 3-4, 91%), Prevotella intermedia (64%; 91%; 100%), Tannerella forsythia (9%; 27%; 54%), Parvimonas micra (18%; 0%; 0%) and Fusobacterium nucleatum (91%; 91%; 82%). Total counts showed statistically significant differences among groups (p=0.019), and grades (p=0.047). There were statistically significant concordant trends between total counts and stages (p=0.005) and grades (p=0.015); and between counts of P. intermedia and T. forsythia and periodontal stages (p=0.029 and p=0.028, respectively). Counts and proportions of Eikenella corrodens and Capnocytophaga spp. showed a statistically significant trend for increasing extension of periodontitis. The odds ratio (OR) for the detection of T. forsythia in periodontitis stages 3 and 4 was greater than in non-periodontitis group (OR= 12; 95% confidence interval >1).
Conclusions: Distinctive profiles of subgingival microbiota could be discerned among stages and grades, since total counts and counts of P. gingivalis and T. forsythia have shown a significant increase as periodontal stages aggravate; and total counts have also shown a significant trend to increase as the rate of progression worsens from slow (A) to rapid (C).