IADR Abstract Archives
Effects of an Adhesive Containing Fluoride-doped Bioglass on Caries-affected Dentine.
Objectives: This in vitro study aimed at evaluating the effects on caries-affected dentine of an experimental adhesive system containing fluoride-doped bioglass, applied in caries-affected dentine (CAD) in self-etching (SE) or etch&rinse (ER) mode.
Methods: Human molars with deep caries lesions were collected and subsequently prepared by means of selective caries excavation using CARISOLV™. The teeth were divided in main groups based on the materials tested in this study and on their mode of application: RMGIC(RIVA LC, SDI); OPT(three-step adhesive, Optibond FL, KERR); UA (universal adhesive Prime&Bond ACTIVE, Dentsply) applied in SE mode (UA-SE) ; EXP-SE (experimental adhesive applied in SE mode) and in ER mode (EXP-ER). EXP consisted in a primer containing remineralisation biomimetic analogues and a bond with 40 wt% fluoride-doped bioglass. UA-SE, RMGIC and OPT were applied and light-cured as per manufacturer’s instructions, while EXP was applied in ER and SE and light-cured for 30s. Bulk-fill composite build up (SDR. Dentsply) was performed in 4mm-layers after adhesive application under simulated pulpal pressure (20cm H2O) . The specimens were then cut in match-sticks (≈1mm2), half of them were stored for 24h and half for 12 months in artificial saliva (AS). They were submitted to μTBS, SEM-EDX mapping and confocal microscopy evaluation.
Results: OPT and UA exhibited the highest bond strength at 24h, while after 1 year, EXP SE was the only adhesive to show no significant reduction in bond strength (p<0.05). The mineralization [EDX: Phosphorous concentration (p<0.05)] was only clearly evident in those specimens treated with the EXP material. Confocal microscopy supported such outcomes showing obvious mineral precipitation at the resin-dentine interface.
Conclusions: Innovative self-etching ion-releasing materials may be necessary to attain a stable bonding and a therapeutic bioactive/biomimetic remineralization of caries affected dentine.
Methods: Human molars with deep caries lesions were collected and subsequently prepared by means of selective caries excavation using CARISOLV™. The teeth were divided in main groups based on the materials tested in this study and on their mode of application: RMGIC(RIVA LC, SDI); OPT(three-step adhesive, Optibond FL, KERR); UA (universal adhesive Prime&Bond ACTIVE, Dentsply) applied in SE mode (UA-SE) ; EXP-SE (experimental adhesive applied in SE mode) and in ER mode (EXP-ER). EXP consisted in a primer containing remineralisation biomimetic analogues and a bond with 40 wt% fluoride-doped bioglass. UA-SE, RMGIC and OPT were applied and light-cured as per manufacturer’s instructions, while EXP was applied in ER and SE and light-cured for 30s. Bulk-fill composite build up (SDR. Dentsply) was performed in 4mm-layers after adhesive application under simulated pulpal pressure (20cm H2O) . The specimens were then cut in match-sticks (≈1mm2), half of them were stored for 24h and half for 12 months in artificial saliva (AS). They were submitted to μTBS, SEM-EDX mapping and confocal microscopy evaluation.
Results: OPT and UA exhibited the highest bond strength at 24h, while after 1 year, EXP SE was the only adhesive to show no significant reduction in bond strength (p<0.05). The mineralization [EDX: Phosphorous concentration (p<0.05)] was only clearly evident in those specimens treated with the EXP material. Confocal microscopy supported such outcomes showing obvious mineral precipitation at the resin-dentine interface.
Conclusions: Innovative self-etching ion-releasing materials may be necessary to attain a stable bonding and a therapeutic bioactive/biomimetic remineralization of caries affected dentine.