IADR Abstract Archives
Dentin Biomodification using Collagen Cross-linkers
Objectives: Dentin biomodification using cross-linkers has been proposed to improve bond durability to dentin. However, literature is not always consistent regarding their effectiveness, especially when the cross-linkers are applied in clinically realistic application times. This study investigated the effect of 2 cross-linkers on the micro-tensile bond strength (μTBS) of 4 adhesives bonded to dentin following either etch&rinse (E&R) or self-etch (SE) bonding modes.
Methods: 80 molars were randomly divided in accordance to the three variables ‘cross-linker’, ‘adhesive’ and ‘bonding mode’ (n=8). The cross-linkers proanthocyanidin (6.5wt%; ‘PAC’; Mega Natural Gold) and 1 ethyl 3 (3 dimethylaminopropyl) carbodiimide HCl (0.3M; ‘EDC’; Fisher Scientific), or distilled water (control), were applied on dentin for 60s after acid-etching (E&R) or priming (SE). The 3-step E&R adhesive OptiBond FL (Kerr), the 2-step SE adhesives Clearfil SE Bond 2 (Kuraray Noritake) and Optibond XTR (Kerr), and the universal adhesive Prime&Bond Active (Dentsply), employed in E&R and SE mode, were applied following the manufacturer’s instructions. Filtek Supreme XTE (3M Oral Care) buildups were made prior to 1-week storage in artificial saliva. After the teeth were sectioned into micro-specimens, sticks were trimmed at the interface to a dumbbell shape with a diameter of 1.1 mm. One half of the specimens were immediately tested (‘immediate’ µTBS), the other half after 100K thermocycles (‘aged’ µTBS). Data were statistically analyzed with 3-way ANOVA (p<.05).
Results: When EDC was applied prior to Clearfil SE Bond 2, a higher μTBS was recorded upon aging than the control. Increased μTBS upon aging was also observed when PAC was applied before Optibond FL. The aged μTBS of Prime&Bond Active, applied both in E&R and SE modes, benefited from both PAC and EDC. However, Optibond XTR was negatively affected by collagen cross linking.
Conclusions: Overall, the benefit of incorporating collagen cross-linkers in the dentin-bonding protocol appeared to depend on the adhesive.
Methods: 80 molars were randomly divided in accordance to the three variables ‘cross-linker’, ‘adhesive’ and ‘bonding mode’ (n=8). The cross-linkers proanthocyanidin (6.5wt%; ‘PAC’; Mega Natural Gold) and 1 ethyl 3 (3 dimethylaminopropyl) carbodiimide HCl (0.3M; ‘EDC’; Fisher Scientific), or distilled water (control), were applied on dentin for 60s after acid-etching (E&R) or priming (SE). The 3-step E&R adhesive OptiBond FL (Kerr), the 2-step SE adhesives Clearfil SE Bond 2 (Kuraray Noritake) and Optibond XTR (Kerr), and the universal adhesive Prime&Bond Active (Dentsply), employed in E&R and SE mode, were applied following the manufacturer’s instructions. Filtek Supreme XTE (3M Oral Care) buildups were made prior to 1-week storage in artificial saliva. After the teeth were sectioned into micro-specimens, sticks were trimmed at the interface to a dumbbell shape with a diameter of 1.1 mm. One half of the specimens were immediately tested (‘immediate’ µTBS), the other half after 100K thermocycles (‘aged’ µTBS). Data were statistically analyzed with 3-way ANOVA (p<.05).
Results: When EDC was applied prior to Clearfil SE Bond 2, a higher μTBS was recorded upon aging than the control. Increased μTBS upon aging was also observed when PAC was applied before Optibond FL. The aged μTBS of Prime&Bond Active, applied both in E&R and SE modes, benefited from both PAC and EDC. However, Optibond XTR was negatively affected by collagen cross linking.
Conclusions: Overall, the benefit of incorporating collagen cross-linkers in the dentin-bonding protocol appeared to depend on the adhesive.