IADR Abstract Archives
Bacteriostatic Effect of Filling Materials Used in Implant-abutment-screw-channel
Objectives: To investigate the bacteriostatic properties of frequently used filling materials in implant-abutment-screw-channel. Secondary objective of this study was to evaluate whether chitosan has bacteriostatic effect.
Methods: Microbial suspension was made dissolving probiotic capsules in hydroionised water. The capsules contained strains of Lactobacillus casei, Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus plantarum, Streptococcus thermophilus, Bifidobacterium bifidum and Bifidobacterium breve. 1ml suspension was spread on blood-agar-plates (BAP). In study one, samples of sterile cotton pellet, prefabricated silver zeolite containing polymerplug, cured temporary cement containing triclosan, a-siliconebased impression material and water-blocking tape consisting of polytetrafluoroethylene and chitosan-filaments derived from a rotary brush, were placed on bacteria-coated BAPs. Four identical plates were included in test group. Three plates with no samples served as negative control, whereas three plates with 2.0% Chlorhexidine served as positive control. All plates were incubated aerobically in 37°and evaluated and photographed after 24, 48 and 72 hours.
In study two, samples from same sealants were stored in 0.5ml hydroionised water and incubated 120hours. The solutions were immersed in filter paper, placed on eight bacteria-covered BAPs and same incubation procedure as first study was repeated. In addition filter paper immersed in 2.0% Chlorhexidine were placed on same BAPs as positive control. Two separate BAPs with no test samples were included as negative control.
Descriptive statistics were performed with SPSS v.25.0 software (SPSS Inc., Chicago, IL, USA).
Results: In both studies no test samples showed bacteria-free zone around filling materials. The same was true for chitosan-containing filaments. All positive controls clearly showed bacteriostatic effect.
Conclusions: Within the limitations of this study, none of the included filling materials nor the chitosan-containing filaments showed bacteriostatic effect.
Methods: Microbial suspension was made dissolving probiotic capsules in hydroionised water. The capsules contained strains of Lactobacillus casei, Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus plantarum, Streptococcus thermophilus, Bifidobacterium bifidum and Bifidobacterium breve. 1ml suspension was spread on blood-agar-plates (BAP). In study one, samples of sterile cotton pellet, prefabricated silver zeolite containing polymerplug, cured temporary cement containing triclosan, a-siliconebased impression material and water-blocking tape consisting of polytetrafluoroethylene and chitosan-filaments derived from a rotary brush, were placed on bacteria-coated BAPs. Four identical plates were included in test group. Three plates with no samples served as negative control, whereas three plates with 2.0% Chlorhexidine served as positive control. All plates were incubated aerobically in 37°and evaluated and photographed after 24, 48 and 72 hours.
In study two, samples from same sealants were stored in 0.5ml hydroionised water and incubated 120hours. The solutions were immersed in filter paper, placed on eight bacteria-covered BAPs and same incubation procedure as first study was repeated. In addition filter paper immersed in 2.0% Chlorhexidine were placed on same BAPs as positive control. Two separate BAPs with no test samples were included as negative control.
Descriptive statistics were performed with SPSS v.25.0 software (SPSS Inc., Chicago, IL, USA).
Results: In both studies no test samples showed bacteria-free zone around filling materials. The same was true for chitosan-containing filaments. All positive controls clearly showed bacteriostatic effect.
Conclusions: Within the limitations of this study, none of the included filling materials nor the chitosan-containing filaments showed bacteriostatic effect.