IADR Abstract Archives
Magnetic Nanocomposite Substrates for Bone Tissue Engineering.
Objectives:
The use of magnetic scaffolds based on polymer matrix and magnetic nanoparticles (MNPs) represents a promising strategy for bone regeneration. The aim of this research was the development of magnetic substrates based on a poly(Ɛ-caprolactone) (PCL) matrix and iron oxide (Fe₃O₄) nanoparticles to enhance hard tissue regeneration.
Methods: Polymeric and nanocomposite substrates were manufactured by processing PCL/Fe₃O₄ (80/20 w/w) pellets through melting and molding. Polymeric PCL substrates were used as control. Appropriate measurements were performed to evaluate morphology, surface topography and magnetic features. Furthermore, cell-laden substrates were stimulated through a discontinuous magnetic field (6 h per day, 20 intervals - 18 min each) to assess the effects of the magnetic field on cell-material interactions. The biological performance of mesenchymal stem cells (hMSCs) seeded on magnetic nanocomposite substrates was evaluated through confocal laser scanning microscopy and AlamarBlue assay to provide qualitative and quantitative information on cell adhesion and viability/proliferation, respectively. Moreover, the ALP/DNA assay was used to evaluate the ability of the nanocomposite substrates to promote osteogenic differentiation. The significance of differences between measurements was evaluated by ANOVA test.
Results: In comparison with polymeric substrates, the results showed a significant increase (p<0.01) of cell adhesion and proliferation for nanocomposite substrates at 14 days from seeding (28.5 % of Alamar blue reduction). With regard to stimulated and unstimulated nanocomposite structures, an early differentiation at 7 days (ALP activity 984.5 ng/[DNA]µg) and a differentiation which was prolonged over time (up to 21 days-1,360.7 ng/[DNA]µg) were observed for nanocomposite magnetically stimulated substrates.
Conclusions:
These findings would seem to be the result of a synergistic effect of the surface chemistry, topography and the application of an external magnetic field. Benefiting from the obtained results, further analyses will be performed on 3D additively manufactured magnetic scaffolds.
The use of magnetic scaffolds based on polymer matrix and magnetic nanoparticles (MNPs) represents a promising strategy for bone regeneration. The aim of this research was the development of magnetic substrates based on a poly(Ɛ-caprolactone) (PCL) matrix and iron oxide (Fe₃O₄) nanoparticles to enhance hard tissue regeneration.
Methods: Polymeric and nanocomposite substrates were manufactured by processing PCL/Fe₃O₄ (80/20 w/w) pellets through melting and molding. Polymeric PCL substrates were used as control. Appropriate measurements were performed to evaluate morphology, surface topography and magnetic features. Furthermore, cell-laden substrates were stimulated through a discontinuous magnetic field (6 h per day, 20 intervals - 18 min each) to assess the effects of the magnetic field on cell-material interactions. The biological performance of mesenchymal stem cells (hMSCs) seeded on magnetic nanocomposite substrates was evaluated through confocal laser scanning microscopy and AlamarBlue assay to provide qualitative and quantitative information on cell adhesion and viability/proliferation, respectively. Moreover, the ALP/DNA assay was used to evaluate the ability of the nanocomposite substrates to promote osteogenic differentiation. The significance of differences between measurements was evaluated by ANOVA test.
Results: In comparison with polymeric substrates, the results showed a significant increase (p<0.01) of cell adhesion and proliferation for nanocomposite substrates at 14 days from seeding (28.5 % of Alamar blue reduction). With regard to stimulated and unstimulated nanocomposite structures, an early differentiation at 7 days (ALP activity 984.5 ng/[DNA]µg) and a differentiation which was prolonged over time (up to 21 days-1,360.7 ng/[DNA]µg) were observed for nanocomposite magnetically stimulated substrates.
Conclusions:
These findings would seem to be the result of a synergistic effect of the surface chemistry, topography and the application of an external magnetic field. Benefiting from the obtained results, further analyses will be performed on 3D additively manufactured magnetic scaffolds.