IADR Abstract Archives
miR-21 and TAC as Salivary Biomarkers for Oral Dysplasia
Objectives: Previous studies have demonstrated that microRNA-21 (miR-21) and total anti-oxidant capacity (TAC) could be potential diagnostic biomarkers for oral squamous cell carcinoma. However, their diagnostic potential in the early stages of carcinogenesis is not clear yet. This study was conducted to determine the salivary levels of miR-21 and TAC in patients with oral hyperplasia and dysplasia.
Methods: We assessed expression of miR-21 and TAC by qPCR in whole unstimulated saliva samples of 30 patients with oral mucosal lesions demonstrating hyperplastic or dysplastic changes and 30 healthy individuals with normal mucosa. Biopsy was taken from the lesions, immediately fixed, then processed and stained with Hematoxylin & Eosin for histopathologic diagnosis. Statistical analysis was performed with IBM® SPSS® (P ≤ 0.05) and ROC curve analysis was performed with MedCalc.
Results:
miR-21 expression was higher in mucosal lesions than normal mucosa and in dysplasia than hyperplasia with significant difference (P < 0.001). TAC expression was lower in mucosal lesions than normal mucosa with significant difference (P = 0.001), and in dysplasia than hyperplasia but with no significant difference (P = 0.093). Diagnostic accuracy, sensitivity and specificity were higher in miR-21 (96.6%, 100%, 94.4%) than TAC (73.3%, 83.3%, 66.7%). Positive predictive value and negative predictive value were higher in miR-21 (92.3%, 100%) than TAC (62.5%, 85.7%). The cut-off value was 9.45 for miR-21 and 20.85 for TAC.
Conclusions:
Oral hyperplasia and dysplasia are associated with altered miR-21 and TAC expression. Salivary miR-21 was more accurate in detecting oral dysplasia than salivary TAC. Salivary miR-21 could be potential diagnostic biomarker for screening and early detection of oral cancer. More studies are required to validate miR-21.
Methods: We assessed expression of miR-21 and TAC by qPCR in whole unstimulated saliva samples of 30 patients with oral mucosal lesions demonstrating hyperplastic or dysplastic changes and 30 healthy individuals with normal mucosa. Biopsy was taken from the lesions, immediately fixed, then processed and stained with Hematoxylin & Eosin for histopathologic diagnosis. Statistical analysis was performed with IBM® SPSS® (P ≤ 0.05) and ROC curve analysis was performed with MedCalc.
Results:
miR-21 expression was higher in mucosal lesions than normal mucosa and in dysplasia than hyperplasia with significant difference (P < 0.001). TAC expression was lower in mucosal lesions than normal mucosa with significant difference (P = 0.001), and in dysplasia than hyperplasia but with no significant difference (P = 0.093). Diagnostic accuracy, sensitivity and specificity were higher in miR-21 (96.6%, 100%, 94.4%) than TAC (73.3%, 83.3%, 66.7%). Positive predictive value and negative predictive value were higher in miR-21 (92.3%, 100%) than TAC (62.5%, 85.7%). The cut-off value was 9.45 for miR-21 and 20.85 for TAC.
Conclusions:
Oral hyperplasia and dysplasia are associated with altered miR-21 and TAC expression. Salivary miR-21 was more accurate in detecting oral dysplasia than salivary TAC. Salivary miR-21 could be potential diagnostic biomarker for screening and early detection of oral cancer. More studies are required to validate miR-21.