IADR Abstract Archives
Air-Abrasion with Bioactive Glass Eradicates S. Mutans Biofilm from Sandblasted and Acid Etched Titanium Surface.
Objectives: To investigate the influence of air - BAG particle abrasion on S. mutans biofilm on sandblasted and acid etched titanium surface.
Methods: Sandblasted and acid etched commercially pure titanium alloy discs were used as substrates for bacteria (n=107). The discs were immersed in S. mutans solution and incubated for 21 h to form S. mutans biofilm. Colonized discs were subjected to air-particle abrasion with the BioglassÒ45S5, one experimental ZnO containing BAG (Zn4) and one inert glass. After the abrasion, the discs were incubated for 5 h in anaerobic chamber followed by assessment of viable S. mutans cells (n=20) and evaluation of the surface morphology using SEM (n=12). Reactivity of the BAG particles was verified in SBF immersion. Thrombogenicity of the glass particle treated discs (n=75) was evaluated spectrophotometrically using whole blood clotting measurement at predetermined time points.
Results: Air-particle abrasion with 45S5 and Zn4 BAGs eradicated S. mutans biofilm. Significantly less viable S. mutans cells were found on discs treated with the BAGs compared to the inert glass (p<0.001). The rise in the pH of the SBF was significantly higher for 45S5 than for Zn4. No significant differences were found in thrombogenicity.
Conclusions: Air-particle abrasion with BAG is effective in eradication of S. mutansbiofilm from sandblasted and acid etched titanium alloy surfaces. Zn4 and 45S5 BAGs had similar biofilm eradicating effects but the Zn4 BAG could be more tissue-friendly and may thus be of benefit for the healing of the tissue.
Methods: Sandblasted and acid etched commercially pure titanium alloy discs were used as substrates for bacteria (n=107). The discs were immersed in S. mutans solution and incubated for 21 h to form S. mutans biofilm. Colonized discs were subjected to air-particle abrasion with the BioglassÒ45S5, one experimental ZnO containing BAG (Zn4) and one inert glass. After the abrasion, the discs were incubated for 5 h in anaerobic chamber followed by assessment of viable S. mutans cells (n=20) and evaluation of the surface morphology using SEM (n=12). Reactivity of the BAG particles was verified in SBF immersion. Thrombogenicity of the glass particle treated discs (n=75) was evaluated spectrophotometrically using whole blood clotting measurement at predetermined time points.
Results: Air-particle abrasion with 45S5 and Zn4 BAGs eradicated S. mutans biofilm. Significantly less viable S. mutans cells were found on discs treated with the BAGs compared to the inert glass (p<0.001). The rise in the pH of the SBF was significantly higher for 45S5 than for Zn4. No significant differences were found in thrombogenicity.
Conclusions: Air-particle abrasion with BAG is effective in eradication of S. mutansbiofilm from sandblasted and acid etched titanium alloy surfaces. Zn4 and 45S5 BAGs had similar biofilm eradicating effects but the Zn4 BAG could be more tissue-friendly and may thus be of benefit for the healing of the tissue.
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